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- W2034697394 abstract "Abstract The hydration of fumarase and the dehydration of l -malate catalysed by fumarase were investigated in water/methanol and water/formamide one phase systems. The effects of the amount of organic solvent on the maximum velocity ( V max ), the Michael is-Menten constant ( K M ) and the equilibrium constant ( K eq ) were studied for both the reaction media. The denaturing power of both methanol and formamide was observed together with the familiar decrease of the K M . Fumarase catalysis in water/methanol systems was further investigated by evaporating the organic solvent and evaluating the degree of reversibility of the inactivation. Reversibility of formamide denaturation was also investigated. The effects of phosphate concentration in the reaction medium with different amounts of methanol was investigated following the variation of the kinetic parameters of the hydration reaction. At high concentrations of phosphate an inhibiting effect appeared. Time-dependent denaturation was also investigated and a remarkable instability of fumarase in systems with percentages ( v/v ) of formamide higher than 10% was observed. 10% formamide proved to be less deactivating than the other non-conventional reaction media so far employed." @default.
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- W2034697394 date "1996-06-01" @default.
- W2034697394 modified "2023-09-27" @default.
- W2034697394 title "Study of fumarase activity in non-conventional media. Part II" @default.
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- W2034697394 doi "https://doi.org/10.1016/1381-1177(95)00016-x" @default.
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