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• W2034707375 abstract "Sonicated calf-thymus DNA (200 ± 30 base pairs) spontaneously forms viscoelastic gels over a wide range of concentration, temperature, and buffer conditions. Quasielastic light scattering (QLS) can be used to monitor this process, because the ratio of dynamic-to-static scattering intensity decreases dramatically as gelation occurs. Using QLS, we have explored the effects of DNA concentration and mono- and divalent cations on the thermal stability of DNA gels. We found that the gel–sol transition temperature (Tgel) varies linearly with [DNA] from 7.5 to 17 mg/mL. Both Na+ and Mg2+ strongly stabilize the gel state. The sharpness of the transition increases with increasing ionic and DNA concentrations. Analysis of the Na+-dependent gelation indicates that the process requires the association of one Na+ per 118 base pairs. Mg2+ effectively stabilizes the gel at concentrations 10-fold below those required for Na+. The unexpectedly large effect of Mg2+ suggests that ion-specific interactions may play an important role in determining gel stability." @default.
• W2034707375 created "2016-06-24" @default.
• W2034707375 creator A5007320411 @default.
• W2034707375 creator A5090557609 @default.
• W2034707375 date "1984-11-01" @default.
• W2034707375 modified "2023-10-16" @default.
• W2034707375 title "DNA gelation in concentrated solutions" @default.
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• W2034707375 doi "https://doi.org/10.1002/bip.360231104" @default.
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