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• W2034711213 abstract "The specific labeling of proteins in living cells using a genetically encodable tag and a small synthetic probe targeting the tag has been craved as an alternative to widely used larger fluorescent proteins. We describe a rapid method with a small tag (21 amino acids) for the fluorescence labeling of cell-surface receptors using a high affinity coiled-coil formation without metals or enzymes. The peptide probes K3 (KIAALKE)3 and K4 (KIAALKE)4 labeled with a fluorophore specifically stained the surface-exposed tag sequence E3 (EIAALEK)3 attached to the N-terminus of the mouse-derived prostaglandin EP3 receptor in living cells (Kd = 64 and 6 nM for K3 and K4, respectively). The labeling was quick (<1 min), nontoxic, and available even in culture medium without affecting receptor function. As an application of this tractable method, the agonist-induced internalization of the human-derived 2-adrenergic receptor and epidermal growth factor receptor was successfully visualized." @default.
• W2034711213 created "2016-06-24" @default.
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• W2034711213 date "2008-06-01" @default.
• W2034711213 modified "2023-10-18" @default.
• W2034711213 title "Coiled-Coil Tag−Probe System for Quick Labeling of Membrane Receptors in Living Cells" @default.
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• W2034711213 doi "https://doi.org/10.1021/cb8000556" @default.
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