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- W2034713613 abstract "Genomic imprinting at the Delta-like 1 (Dlk1)-Maternally expressed gene 3 (Meg3) locus is regulated by the Meg3 differentially methylated region (DMR), but the mechanism by which this DMR acts is unknown. The goal of this study was to analyze the Meg3 DMR during imprinting establishment and maintenance for the presence of histone modifications and trans-acting DNA binding proteins using chromatin immunoprecipitation. In embryonic stem (ES) cells, where Meg3 is biallelically expressed, the DMR showed variable DNA methylation, with biallelic methylation at one region but paternal allele-specific methylation at another. All histone modifications detected at the Meg3 DMR of ES cells were biallelic. In embryonic day 12.5 (e12.5) embryos, where Meg3 is maternally expressed, the paternal Meg3 DMR was methylated, and activating histone modifications were specific to the maternal DMR. DNA-binding proteins that represent potential regulatory factors were identified in both ES cells and embryos." @default.
- W2034713613 created "2016-06-24" @default.
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- W2034713613 date "2012-09-01" @default.
- W2034713613 modified "2023-10-18" @default.
- W2034713613 title "Identification of imprinting regulators at the Meg3 differentially methylated region" @default.
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- W2034713613 doi "https://doi.org/10.1016/j.ygeno.2012.06.001" @default.
- W2034713613 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3428518" @default.
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