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• W2034721868 endingPage "28" @default.
• W2034721868 startingPage "15" @default.
• W2034721868 abstract "Bovine serum was used to identify a recombinant phage clone carrying the Pasteurella haemolytica leukotoxin gene. This fragment produced the 102-kD leukotoxin and several smaller P. haemolytica-specific protein antigens in Escherichia coli. An additional contiguous fragment, containing sequences upstream from the leukotoxin gene, was isolated from a plasmid library with a probe specific for the region near the 5′ end of the leukotoxin gene. Using these clones, we determined the nucleotide sequence of a 7745-bp region that included four open reading frames: an upstream gene, lktC; the leukotoxin gene, lktA; and two down-stream genes, lktB, and lktD. The predicted molecular weights of the proteins encoded by these genes were 19.9, 102, 79.6, and 54.7 kD, respectively. These genes and their predicted proteins were similar in organization and in sequence to the corresponding elements of the gene cluster that encodes an E. coli α-hemolysin and its activation and secretion functions. Expression of the leukotoxin was enhanced in E. coli, by fusing the gene to the lac promoter. Under these conditions the leukotoxin was not secreted into the medium, as it is in P. haemolytica. However, in the presence of the α-hemolysin genes, the leukotoxin was secreted into the medium, demonstrating functional complementation by the hemolysin secretory system." @default.
• W2034721868 created "2016-06-24" @default.
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• W2034721868 date "1989-01-01" @default.
• W2034721868 modified "2023-10-11" @default.
• W2034721868 title "DNA Sequence of the<i>Pasteurella haemolytica</i>Leukotoxin Gene Cluster" @default.
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• W2034721868 doi "https://doi.org/10.1089/dna.1.1989.8.15" @default.
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