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W2034726027 abstract "V uvedenĂŠ prĂĄci byla sledovĂĄna odezva biochemickĂ˝ch markerĹŻ u ryb na toxickĂŠ pĹŻsobenĂ cyanobakteriĂĄlnĂch toxinĹŻ (microcystinĹŻ). Mezi nejsledovanÄjĹĄĂ biomarkery vlivu cyanobacteriĂĄlnĂch toxinĹŻ paĹĂ parametry oxidativnĂho stresu - glutation S-transferĂĄza (GST), neenzymatickĂ˝ antioxidant glutation (GSH), superoxid dismutĂĄza (SOD), katalĂĄza (CAT), glutation peroxidĂĄza (GPx), lipidovĂĄ peroxidace (LPO), malondialdehyd (MDA), glutation reduktĂĄza (GR), parametry krve - hodnota hemoglobinu (Hb), hematokryt (PCV), stĹednĂ barevnĂĄ koncentrace (MCHC), stĹednĂ objem erytrocytu (MCV), stĹednĂ obsah hemoglobinu erytrocytu (MCH), poÄet erytrocytĹŻ (RBC) poÄet leukocytĹŻ (WBC), parametry krevnĂ plazmy - alanin aminotransferĂĄza (ALT), aspartĂĄt aminotransferĂĄza (AST), laktĂĄt dehydrogenĂĄza (LDH), alkalickĂĄ fosfatĂĄza (ALP), cholinesterĂĄza (CHE), celkovĂŠ bĂlkoviny (TP), glukĂłza (GLU), laktĂĄt (LACT), Ĺželezo (Fe), vĂĄpnĂk (Ca), hoĹÄĂk (Mg), celkovĂ˝ bilirubin (BIL), fosfor (P) a aktivita protein fosfatĂĄz (PP1, PP2A).BiochemickĂŠ parametry krve a plazmy u ryb jsou ovlivnÄny mnoha endogennĂmi i exogennĂmi faktory. Mezi nejsledovanÄjĹĄĂ parametry patĹĂ enzymy krevnĂ plazmy (ALT, AST and LDH), kterĂŠ vĂ˝raznÄ zvyĹĄujĂ svoji aktivitu po intraperitoneĂĄlnĂ nebo perorĂĄlnĂ aplikaci microcystinĹŻ rybĂĄm. ToxickĂ˝ ĂşÄinek sinic na ryby v pĹĂrodnĂm prostĹedĂ je vĂ˝raznÄ niĹžĹĄĂ neĹž po pĹĂmĂŠ aplikaci toxinu do rybĂho organismu a zmÄny v aktivitÄ enzymĹŻ u tÄchto pĹĂrodnĂch experimentĹŻ jsou vÄtĹĄinou neprĹŻkaznĂŠ. Na vĂ˝znamu pak nabĂ˝vajĂ dalĹĄĂ parametry krevnĂ plazmy indikujĂcĂ negativnĂ pĹŻsobenĂ sinic (elektrolyty, laktĂĄt, glukĂłza, albumin, cholesterol aj.).ToxickĂŠ metabolity sinic vyvolĂĄvajĂ oxidativnĂ stres a sledovĂĄnĂ zmÄn vhodnĂ˝ch biomarkerĹŻ lze dobĹe vyuĹžĂt takĂŠ u ryb k ÄasnĂŠ indikaci poĹĄkozenĂ organismu v dĹŻsledku expozice toxickĂ˝m sinicĂm. Je zĹejmĂĄ silnĂĄ ÄasovĂĄ zĂĄvislost modulace detoxikaÄnĂch pochodĹŻ pĹi vystavenĂ ryb vlivu microcystinĹŻ. V zĂĄvislosti na dĂŠlce expozice tak lze vysledovat nĂĄrĹŻst i pokles aktivity jednotlivĂ˝ch biomarkerĹŻ oxidativnĂho stresu zpĹŻsobenĂ˝ stimulacĂ nebo inhibicĂ protektivnĂch procesĹŻ. VĂ˝raznĂŠ rozdĂly lze v zĂĄvislosti na intenzitÄ detoxifikaÄnĂch pochodĹŻ vysledovat i u rĹŻznĂ˝ch orgĂĄnĹŻ ryb, rovnÄĹž tak jsou nalĂŠzĂĄny vĂ˝raznĂŠ rozdĂly mezi jednotlivĂ˝mi druhy ryb." @default.
W2034726027 created "2016-06-24" @default.
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W2034726027 date "2014-11-03" @default.
W2034726027 modified "2023-09-26" @default.
W2034726027 title "Biochemical indices are modulated in fish exposed to cyanobacterial toxins (microcystins)" @default.
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W2034726027 doi "https://doi.org/10.11118/actaun200856050255" @default.
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