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- W2034753089 abstract "A rapid radiometric assay for measuring oxygen insensitive methyl red azoreductase has been developed. [14C]Methyl red was synthesized from [U-14C]aniline; the final product had a radiochemical purity of ≥98%. [14C]Methyl red was then used as the substrate to assay azoreductase activity using a modification of a previously published procedure. Results obtained by the radiometric assay were comparable to those obtained using the fluorescent procedure. The radiometric assay is quick, simple, and reliable. Methyl red azoreductase has been shown to be identical with DT-diaphorase [EC 1.6.99.2]. The assay described can therefore be used to assay DT-diaphorase activity and does not suffer from the limitations, such as lack of specificity and low sensitivity, usually associated with DT-diaphorase assays." @default.
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- W2034753089 date "1981-04-01" @default.
- W2034753089 modified "2023-10-09" @default.
- W2034753089 title "A radiometric assay for methyl red azoreductase DT-diaphorase [EC 1.6.99.2]" @default.
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- W2034753089 doi "https://doi.org/10.1016/0003-2697(81)90297-9" @default.
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