FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034755997> ?p ?o ?g. }

• W2034755997 endingPage "1422" @default.
• W2034755997 startingPage "1415" @default.
• W2034755997 abstract "ABSTRACT Type IV secretion systems mediate conjugative plasmid transfer as well as the translocation of virulence factors from various gram-negative pathogens to eukaryotic host cells. The translocation apparatus consists of 9 to 12 components, and the components from different organisms are believed to have similar functions. However, orthologs to proteins of the prototypical type IV system, VirB of Agrobacterium tumefaciens , typically share only 15 to 30% identical amino acids, and functional complementation between components of different type IV secretion systems has not been achieved. We here report a heterologous complementation in the case of A. tumefaciens virB1 defects with its orthologs from Brucella suis (VirB1s) and the IncN plasmid pKM101 (TraL). In contrast, expression of the genes encoding the VirB1 orthologs from the IncF plasmid (open reading frame 169) and from the Helicobacter pylori cag pathogenicity island (HP0523) did not complement VirB1 functions. The complementation of VirB1 activity was assessed by T-pilus formation, by tumor formation on wounded plants, by IncQ plasmid transfer, and by IncQ plasmid recipient assay. Replacement of the key active-site Glu residue by Ala abolished the complementation by VirB1 from B. suis and by TraL, demonstrating that heterologous complementation requires an intact lytic transglycosylase active site. In contrast, the VirB1 active-site mutant from A. tumefaciens retained considerable residual activity in various activity assays, implying that this protein exerts additional effects during the type IV secretion process." @default.
• W2034755997 created "2016-06-24" @default.
• W2034755997 creator A5016478729 @default.
• W2034755997 creator A5041207516 @default.
• W2034755997 creator A5054176992 @default.
• W2034755997 creator A5062681491 @default.
• W2034755997 creator A5070753660 @default.
• W2034755997 date "2004-03-01" @default.
• W2034755997 modified "2023-09-27" @default.
• W2034755997 title "VirB1 Orthologs from <i>Brucella suis</i> and pKM101 Complement Defects of the Lytic Transglycosylase Required for Efficient Type IV Secretion from <i>Agrobacterium tumefaciens</i>" @default.
• W2034755997 cites W1508806860 @default.
• W2034755997 cites W1511963303 @default.
• W2034755997 cites W1546895016 @default.
• W2034755997 cites W1552257323 @default.
• W2034755997 cites W1592892951 @default.
• W2034755997 cites W1808770289 @default.
• W2034755997 cites W1833389385 @default.
• W2034755997 cites W1855475827 @default.
• W2034755997 cites W1882245944 @default.
• W2034755997 cites W1911630083 @default.
• W2034755997 cites W1975085160 @default.
• W2034755997 cites W1990657715 @default.
• W2034755997 cites W1993660875 @default.
• W2034755997 cites W2000585831 @default.
• W2034755997 cites W2002909586 @default.
• W2034755997 cites W2006865177 @default.
• W2034755997 cites W2007276966 @default.
• W2034755997 cites W2007330933 @default.
• W2034755997 cites W2021743399 @default.
• W2034755997 cites W2028402847 @default.
• W2034755997 cites W2028622989 @default.
• W2034755997 cites W2043219019 @default.
• W2034755997 cites W2043822711 @default.
• W2034755997 cites W2068518785 @default.
• W2034755997 cites W2076556358 @default.
• W2034755997 cites W2082720444 @default.
• W2034755997 cites W2087327203 @default.
• W2034755997 cites W2092900120 @default.
• W2034755997 cites W2100837269 @default.
• W2034755997 cites W2103341064 @default.
• W2034755997 cites W2103937037 @default.
• W2034755997 cites W2106764265 @default.
• W2034755997 cites W2110132014 @default.
• W2034755997 cites W2110220326 @default.
• W2034755997 cites W2110566743 @default.
• W2034755997 cites W2114456096 @default.
• W2034755997 cites W2115069651 @default.
• W2034755997 cites W2116263464 @default.
• W2034755997 cites W2119483869 @default.
• W2034755997 cites W2124092500 @default.
• W2034755997 cites W2125718756 @default.
• W2034755997 cites W2127847106 @default.
• W2034755997 cites W2138821613 @default.
• W2034755997 cites W2140916275 @default.
• W2034755997 cites W2142276846 @default.
• W2034755997 cites W2143649761 @default.
• W2034755997 cites W2147512361 @default.
• W2034755997 cites W2155656806 @default.
• W2034755997 cites W2157528992 @default.
• W2034755997 cites W2159509595 @default.
• W2034755997 cites W2160084442 @default.
• W2034755997 cites W2164800766 @default.
• W2034755997 doi "https://doi.org/10.1128/jb.186.5.1415-1422.2004" @default.
• W2034755997 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/344403" @default.
• W2034755997 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14973016" @default.
• W2034755997 hasPublicationYear "2004" @default.
• W2034755997 type Work @default.
• W2034755997 sameAs 2034755997 @default.
• W2034755997 citedByCount "51" @default.
• W2034755997 countsByYear W20347559972012 @default.
• W2034755997 countsByYear W20347559972013 @default.
• W2034755997 countsByYear W20347559972014 @default.
• W2034755997 countsByYear W20347559972015 @default.
• W2034755997 countsByYear W20347559972017 @default.
• W2034755997 countsByYear W20347559972018 @default.
• W2034755997 countsByYear W20347559972020 @default.
• W2034755997 countsByYear W20347559972021 @default.
• W2034755997 countsByYear W20347559972022 @default.
• W2034755997 countsByYear W20347559972023 @default.
• W2034755997 crossrefType "journal-article" @default.
• W2034755997 hasAuthorship W2034755997A5016478729 @default.
• W2034755997 hasAuthorship W2034755997A5041207516 @default.
• W2034755997 hasAuthorship W2034755997A5054176992 @default.
• W2034755997 hasAuthorship W2034755997A5062681491 @default.
• W2034755997 hasAuthorship W2034755997A5070753660 @default.
• W2034755997 hasBestOaLocation W20347559971 @default.
• W2034755997 hasConcept C104317684 @default.
• W2034755997 hasConcept C143065580 @default.
• W2034755997 hasConcept C182615771 @default.
• W2034755997 hasConcept C188082640 @default.
• W2034755997 hasConcept C204241405 @default.
• W2034755997 hasConcept C22744801 @default.
• W2034755997 hasConcept C2522874641 @default.
• W2034755997 hasConcept C2776162087 @default.
• W2034755997 hasConcept C2779656422 @default.
• W2034755997 hasConcept C2780115692 @default.
• W2034755997 hasConcept C49039625 @default.
• W2034755997 hasConcept C54355233 @default.

• next