FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034756072> ?p ?o ?g. }

• W2034756072 endingPage "1055" @default.
• W2034756072 startingPage "1049" @default.
• W2034756072 abstract "A recombinant chimeric plasminogen activator (f beta/scuPA-32k), with a fibrin beta-chain peptide (comprising Gly15 through Arg 42) linked to the N-terminal of a low molecular mass (32 kDa) single-chain urokinase (scuPA-32k, comprising Leu144 through Leu 411) via a 50 amino acid linker sequence, was produced by expression the corresponding chimeric cDNA in Escherichia coli cells. After refolding in vitro, the chimeric protein was purified to homogeneity by zinc chelate-Sepharose chromatography, Sephacryl S200 chromatography and benzamidine-Sepharose chromatography in sequence. The apparent molecular mass was 36 kDa shown by SDS-PAGE analysis. The special activity was 87,000 IU/mg detected by fibrin plate determination. F beta/scuPA-32k could directly activate plasminogen following Michaelis-Menten kinetics with K(m) = 0.52 microM and k(2) = 0.0024 s(-1). Mediated by plasmin, the single-chain molecule could be converted to the active two-chain molecule. The chimeric protein had 3.3 times higher fibrin affinity than scuPA-32k in the fibrin concentration of 3.2 mg/mL, while the chimeric protein inhibited the fibrin clotting and platelet aggregation. F beta/scuPA-32k showed a higher thrombolytic potency in vitro plasma clot lysis than scuPA-32k and depleted less fibrinogen in plasma. These results showed that the chimeric protein had not only higher fibrinolytic activity but also anti-thrombus activity. Further evaluation of the thrombolytic potential in appropriate animal models is required." @default.
• W2034756072 created "2016-06-24" @default.
• W2034756072 creator A5013345797 @default.
• W2034756072 creator A5061883017 @default.
• W2034756072 creator A5084677690 @default.
• W2034756072 date "2001-11-01" @default.
• W2034756072 modified "2023-09-25" @default.
• W2034756072 title "Construction and characterization of a recombinant chimeric plasminogen activator consisting of a fibrin peptide and a low molecular mass single-chain urokinase" @default.
• W2034756072 cites W1485376061 @default.
• W2034756072 cites W1495467917 @default.
• W2034756072 cites W1524135583 @default.
• W2034756072 cites W1557927258 @default.
• W2034756072 cites W1580322940 @default.
• W2034756072 cites W174720846 @default.
• W2034756072 cites W1968135936 @default.
• W2034756072 cites W2003364720 @default.
• W2034756072 cites W2009942259 @default.
• W2034756072 cites W2021437817 @default.
• W2034756072 cites W2031324615 @default.
• W2034756072 cites W2043352260 @default.
• W2034756072 cites W2079409933 @default.
• W2034756072 cites W2081576889 @default.
• W2034756072 cites W2084518893 @default.
• W2034756072 cites W2093656319 @default.
• W2034756072 cites W2100837269 @default.
• W2034756072 cites W2101108802 @default.
• W2034756072 cites W2123199591 @default.
• W2034756072 cites W2133152633 @default.
• W2034756072 cites W2135846300 @default.
• W2034756072 cites W2138270253 @default.
• W2034756072 cites W2148477942 @default.
• W2034756072 cites W40278976 @default.
• W2034756072 cites W4293247451 @default.
• W2034756072 doi "https://doi.org/10.1016/s0300-9084(01)01352-9" @default.
• W2034756072 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11879733" @default.
• W2034756072 hasPublicationYear "2001" @default.
• W2034756072 type Work @default.
• W2034756072 sameAs 2034756072 @default.
• W2034756072 citedByCount "4" @default.
• W2034756072 countsByYear W20347560722013 @default.
• W2034756072 crossrefType "journal-article" @default.
• W2034756072 hasAuthorship W2034756072A5013345797 @default.
• W2034756072 hasAuthorship W2034756072A5061883017 @default.
• W2034756072 hasAuthorship W2034756072A5084677690 @default.
• W2034756072 hasConcept C104317684 @default.
• W2034756072 hasConcept C123894998 @default.
• W2034756072 hasConcept C134018914 @default.
• W2034756072 hasConcept C153911025 @default.
• W2034756072 hasConcept C167625842 @default.
• W2034756072 hasConcept C179247698 @default.
• W2034756072 hasConcept C181199279 @default.
• W2034756072 hasConcept C185592680 @default.
• W2034756072 hasConcept C203014093 @default.
• W2034756072 hasConcept C2776892390 @default.
• W2034756072 hasConcept C2779281246 @default.
• W2034756072 hasConcept C2779679481 @default.
• W2034756072 hasConcept C2779881004 @default.
• W2034756072 hasConcept C2781071845 @default.
• W2034756072 hasConcept C40767141 @default.
• W2034756072 hasConcept C54173615 @default.
• W2034756072 hasConcept C54355233 @default.
• W2034756072 hasConcept C55493867 @default.
• W2034756072 hasConcept C58121356 @default.
• W2034756072 hasConcept C86803240 @default.
• W2034756072 hasConceptScore W2034756072C104317684 @default.
• W2034756072 hasConceptScore W2034756072C123894998 @default.
• W2034756072 hasConceptScore W2034756072C134018914 @default.
• W2034756072 hasConceptScore W2034756072C153911025 @default.
• W2034756072 hasConceptScore W2034756072C167625842 @default.
• W2034756072 hasConceptScore W2034756072C179247698 @default.
• W2034756072 hasConceptScore W2034756072C181199279 @default.
• W2034756072 hasConceptScore W2034756072C185592680 @default.
• W2034756072 hasConceptScore W2034756072C203014093 @default.
• W2034756072 hasConceptScore W2034756072C2776892390 @default.
• W2034756072 hasConceptScore W2034756072C2779281246 @default.
• W2034756072 hasConceptScore W2034756072C2779679481 @default.
• W2034756072 hasConceptScore W2034756072C2779881004 @default.
• W2034756072 hasConceptScore W2034756072C2781071845 @default.
• W2034756072 hasConceptScore W2034756072C40767141 @default.
• W2034756072 hasConceptScore W2034756072C54173615 @default.
• W2034756072 hasConceptScore W2034756072C54355233 @default.
• W2034756072 hasConceptScore W2034756072C55493867 @default.
• W2034756072 hasConceptScore W2034756072C58121356 @default.
• W2034756072 hasConceptScore W2034756072C86803240 @default.
• W2034756072 hasIssue "11-12" @default.
• W2034756072 hasLocation W20347560721 @default.
• W2034756072 hasLocation W20347560722 @default.
• W2034756072 hasOpenAccess W2034756072 @default.
• W2034756072 hasPrimaryLocation W20347560721 @default.
• W2034756072 hasRelatedWork W1999263593 @default.
• W2034756072 hasRelatedWork W2000512540 @default.
• W2034756072 hasRelatedWork W2021156020 @default.
• W2034756072 hasRelatedWork W2072966615 @default.
• W2034756072 hasRelatedWork W2076378797 @default.
• W2034756072 hasRelatedWork W2085566034 @default.
• W2034756072 hasRelatedWork W2105699409 @default.
• W2034756072 hasRelatedWork W2276908954 @default.
• W2034756072 hasRelatedWork W2375409019 @default.

• next