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- W2034769731 abstract "ABSTRACT Biosensor designs are emerging at a significant rate and play an increasingly important role in foodborne pathogen detection. Conducting polymers are excellent tools for the fabrication of biosensors and polypyrrole has been used in the detection of biomolecules due to its unique properties. The pr ime intention of this paper was to pioneer the design and fabrication of a single-strand (ss) DNA biosensor for the detection of the Bacillus cereus (B.cereus ) group species. Growth of B. cereus, results in production of several highly active toxins. Therefore, consumption of food containing >10 6 bacteria/gm may results in emetic and diarrhoeal syndromes. The most common source of this bacterium is found in liquid food products, milk powder, mixed food products and is of particular concern in the baby formula industry. The electrochemical deposition technique, such as cyclic voltammetry, was used to develop and test a model DNA-based biosensor on a gold electrode electropoly merized with polypyrrole. The electrica lly conducting polymer, polypyrrole is used as a platform for immobilizing DN A (1µg) on the gold electrode surface, si nce it can be more easily deposited from neutral pH aqueous solutions of pyrrolemonomers. The average current peak during the electrodeposition event is 288µA. There is a clear change in the current after hybridization of the comple mentary oligonucleotid e (6.35µA) and for the noncomplementary oligonucleotide (5.77µA). The drop in current after each event was clearly noticeable and it proved to be effective. Keywords: Bacillus cereus group spp; foodborne pathogen detection; DNA; cyclic voltammetry; biosensor; conducting polymers." @default.
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- W2034769731 date "2009-05-01" @default.
- W2034769731 modified "2023-09-23" @default.
- W2034769731 title "Conducting polymer based DNA biosensor for the detection of the Bacillus cereus group species" @default.
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- W2034769731 doi "https://doi.org/10.1117/12.818631" @default.
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