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• W2034776719 endingPage "884" @default.
• W2034776719 startingPage "879" @default.
• W2034776719 abstract "In order to take full advantage of the industrially important yeast Candida utilis, we developed a practical recombinant DNA tool for multiple gene disruption in C. utilis based on the Cre-loxP system, which makes possible the reuse of selection markers. For this purpose, two plasmids were constructed: one harbored a heterologous loxP-flanked selection marker cassette carrying the gene responsible for hygromycin B-resistance, and the other had an autonomous replication sequence (ARS) and a Cre-recombinase expression module. Multiple disruption of C. utilis NBRC0988 URA3 genes (CuURA3), encoding orotidine-5'-phosphate decarboxylase, validated the efficiency of the system. The fourth round of deletion yielded a null mutant, i.e., a uracil auxotroph, giving some support to the possibility that C. utilis NBRC0988 is a tetraploid. This agreed very well with the outcomes of FACS analysis, which showed that various strains of this yeast contained 3-5 times more DNA than a Saccharomyces cerevisiae haploid." @default.
• W2034776719 created "2016-06-24" @default.
• W2034776719 creator A5015354792 @default.
• W2034776719 creator A5042372860 @default.
• W2034776719 creator A5079924769 @default.
• W2034776719 date "2009-04-23" @default.
• W2034776719 modified "2023-10-17" @default.
• W2034776719 title "Efficient Gene Disruption in the High-Ploidy Yeast<i>Candida utilis</i>Using the Cre-<i>loxP</i>System" @default.
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• W2034776719 doi "https://doi.org/10.1271/bbb.80799" @default.
• W2034776719 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19352042" @default.
• W2034776719 hasPublicationYear "2009" @default.
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