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• W2034779239 abstract "Background: Melastatin (TRPM1), a.k.a. transient receptor potential cation channel, subfamily M, member 1 (TRPM‐1) regulates melanocyte differentiation and proliferation. TRPM1 is transcriptionally regulated by the essential melanocyte transcription factor MITF (microphthalmia‐associated transcription factor). For the most part, MITF expression is preserved during melanoma progression, while TRPM1 mRNA expression decreases or is completely lost. The loss of TRPM1 is associated with melanomas that are more aggressive. Objective: To assess the relationship between TRPM1 mRNA expression and the expression of MITF and nine other markers of melanocytes and melanin‐related proteins by immunohistochemistry in normal skin, scars, hair follicles and ordinary melanocytic nevi. Methods: Samples of normal skin (n = 102; from tumor excisions and plastic procedures), scars (n = 5; from re‐excision specimens) and compound melanocytic nevi (n = 4) were evaluated for the presence of TRPM1 mRNA transcripts as detected by chromogenic in situ hybridization (CISH). Immunohistochemical techniques were used to detect melanin‐related proteins including: MITF, S100 protein, Mart‐1, tyrosinase, Mel5, HMB45, tyrosinase‐related protein‐1 (TRP1), TRP2 and α‐melanocyte stimulating hormone (αMSH). The labeling index (LI) was defined as the number of intraepidermal cells expressing mRNA or protein per one hundred basal keratinocytes. Results: A wide range of LI was found for all markers (0–33 positive cells/100 keratinocytes). When these LI were compared, no significant differences in the expression of MITF, S100, Mart1, tyrosinase proteins and TRPM1 mRNA were identified. The LI for TRPM1 mRNA expression ranged from 74% of that for MITF to 86% for tyrosinase. The LI for TRP‐1, TRP‐2 and Mel5 was similar to that of TRPM1, while HMB‐45 had a significantly lower LI than all other markers. TRPM1 mRNA correlated most tightly with MITF and tyrosinase expression (r = 0.81 and 0.68, respectively, both p = 0.0001). Likewise, the strongest correlation among all the melanin‐related proteins existed between tyrosinase and MITF (r = 0.79, p = 0.0001). There was variable expression of melanin‐related proteins when LI were analyzed by anatomic site, patient age, extent of sun‐damage and proximity to a melanocytic tumor. Anogenital skin showed the highest and acral skin the lowest LI for TRPM1, MITF, S100 protein, Tyrosinase, Mel5 and HMB45. Advanced age (> 60 years) was associated with decreased TRPM1 expression. Sun‐damaged skin exhibited significantly increased LI as measured by MITF, S100 protein, Mart1, tyrosinase and HMB‐45, but no differences for TRPM1. However, the MITF‐TRPM1 differential (i.e. MITF LI‐TRPM1 LI = MITF+TRPM1 − melanocytes) was significantly increased in site‐matched skin (4.6 ± 4.4 vs. 1.5 ± 2.5, p = 0.01). There was a suggestion of reduced LI in normal skin in the proximity of melanoma (from melanoma re‐excision specimens) for S100, HMB45 and TRPM1 mRNA. TRPM1 LI was significantly decreased in scars compared to normal skin (5.6 ± 1.4 vs. 9.7 ± 4.3, p = 0.02), this was reflected in an increase in the MITF‐TRPM1 differential (9.6 ± 7.5 vs. 3.2 ± 3.1, p = 0.0001). MITF LI were consistently higher than MSLN LI at all levels of the hair follicle; notably, MITF was expressed by isthmic‐bulge cells. In ordinary melanocytic nevi, MITF and TRPM1 expression decreased with melanocyte descent: there was more signal for both markers in superficial epithelioid type A melanocytes than deeper type C melanocytes. Conclusions: By CISH, TRPM1 mRNA expression is specific for melanocytes and strongly associated with MITF and tyrosinase expression, the latter implicating a mature melanocyte phenotype. However, in normal skin, TRPM1 mRNA expression appears to be dynamic, labeling most but not all melanocytes, with variable expression ostensibly related to local environmental factors. Lu S, Slominski A, Yang S‐E, Sheehan C, Ross J, Carlson JA. TRPM1 mRNA expression in normal skin melanocytes." @default.
• W2034779239 created "2016-06-24" @default.
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• W2034779239 date "2010-03-19" @default.
• W2034779239 modified "2023-09-23" @default.
• W2034779239 title "The correlation of TRPM1 (Melastatin) mRNA expression with microphthalmia-associated transcription factor (MITF) and other melanogenesis-related proteins in normal and pathological skin, hair follicles and melanocytic nevi" @default.
• W2034779239 cites W1493098692 @default.
• W2034779239 cites W1536967003 @default.
• W2034779239 cites W1581483600 @default.
• W2034779239 cites W1607261526 @default.
• W2034779239 cites W1831946729 @default.
• W2034779239 cites W1967422081 @default.
• W2034779239 cites W1967465054 @default.
• W2034779239 cites W1969662781 @default.
• W2034779239 cites W1970911811 @default.
• W2034779239 cites W1971717315 @default.
• W2034779239 cites W1972031617 @default.
• W2034779239 cites W1972206478 @default.
• W2034779239 cites W1972254125 @default.
• W2034779239 cites W1978010855 @default.
• W2034779239 cites W1982635732 @default.
• W2034779239 cites W1987202097 @default.
• W2034779239 cites W1987746356 @default.
• W2034779239 cites W1988407228 @default.
• W2034779239 cites W1991949008 @default.
• W2034779239 cites W2000467213 @default.
• W2034779239 cites W2003831501 @default.
• W2034779239 cites W2004988071 @default.
• W2034779239 cites W2005947606 @default.
• W2034779239 cites W2012258129 @default.
• W2034779239 cites W2015009944 @default.
• W2034779239 cites W2015897989 @default.
• W2034779239 cites W2019143958 @default.
• W2034779239 cites W2033182545 @default.
• W2034779239 cites W2035913145 @default.
• W2034779239 cites W2036500143 @default.
• W2034779239 cites W2043584813 @default.
• W2034779239 cites W2046756819 @default.
• W2034779239 cites W2048126835 @default.
• W2034779239 cites W2053583111 @default.
• W2034779239 cites W2053665918 @default.
• W2034779239 cites W2054868496 @default.
• W2034779239 cites W2063161685 @default.
• W2034779239 cites W2066545172 @default.
• W2034779239 cites W2068157074 @default.
• W2034779239 cites W2068526230 @default.
• W2034779239 cites W2072657606 @default.
• W2034779239 cites W2074961267 @default.
• W2034779239 cites W2077593685 @default.
• W2034779239 cites W2081527131 @default.
• W2034779239 cites W2085340320 @default.
• W2034779239 cites W2087988834 @default.
• W2034779239 cites W2092129054 @default.
• W2034779239 cites W2097824035 @default.
• W2034779239 cites W2098430250 @default.
• W2034779239 cites W2106771547 @default.
• W2034779239 cites W2122505959 @default.
• W2034779239 cites W2129131734 @default.
• W2034779239 cites W2132020248 @default.
• W2034779239 cites W2134449270 @default.
• W2034779239 cites W2144200294 @default.
• W2034779239 cites W2151432329 @default.
• W2034779239 cites W2160075129 @default.
• W2034779239 cites W2169209908 @default.
• W2034779239 cites W2322448842 @default.
• W2034779239 cites W2424408121 @default.
• W2034779239 cites W2485139893 @default.
• W2034779239 cites W4230220892 @default.
• W2034779239 cites W4252241660 @default.
• W2034779239 cites W4254298360 @default.
• W2034779239 cites W4384261515 @default.
• W2034779239 doi "https://doi.org/10.1111/j.1600-0560.2010.01504.x" @default.
• W2034779239 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2882198" @default.
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