Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034779712> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2034779712 endingPage "205" @default.
- W2034779712 startingPage "197" @default.
- W2034779712 abstract "A functional polyacrylic acid (PAA) adsorbent has been prepared for metal chelate affinity chromatography. It has been found to chelate nickel ion Ni2+ strongly, and was evaluated for the ability to bind proteins containing neighbouring histidine residues. The principle of the technique was illustrated with Aeromonas hydrophila outer membrane protein OmpTS. DNA elements coding for adjacent histidines were fused to the Aeromonas hydrophila ompTS gene. Subsequent expression in E. coli resulted in the production of hybrid protein His6-OmpTS that could be purified by Ni2+-PAA affinity chromatography. The remarkable specificity found makes it an attractive addition to the range of adsorbents for metal chelate affinity chromatography." @default.
- W2034779712 created "2016-06-24" @default.
- W2034779712 creator A5011351573 @default.
- W2034779712 creator A5022455503 @default.
- W2034779712 creator A5050681210 @default.
- W2034779712 creator A5079620576 @default.
- W2034779712 date "2001-07-01" @default.
- W2034779712 modified "2023-09-27" @default.
- W2034779712 title "Functional polymer affinity matrix for purifying hexahistidine-tagged recombinant protein" @default.
- W2034779712 cites W1970893465 @default.
- W2034779712 cites W1974224727 @default.
- W2034779712 cites W1982652982 @default.
- W2034779712 cites W1990607884 @default.
- W2034779712 cites W1994623738 @default.
- W2034779712 cites W2007491605 @default.
- W2034779712 cites W2051290111 @default.
- W2034779712 cites W2059674598 @default.
- W2034779712 cites W2070543174 @default.
- W2034779712 cites W2074969682 @default.
- W2034779712 cites W2076224771 @default.
- W2034779712 cites W2100837269 @default.
- W2034779712 cites W2214301671 @default.
- W2034779712 doi "https://doi.org/10.1016/s0021-9673(01)00863-9" @default.
- W2034779712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11471803" @default.
- W2034779712 hasPublicationYear "2001" @default.
- W2034779712 type Work @default.
- W2034779712 sameAs 2034779712 @default.
- W2034779712 citedByCount "6" @default.
- W2034779712 countsByYear W20347797122012 @default.
- W2034779712 countsByYear W20347797122014 @default.
- W2034779712 crossrefType "journal-article" @default.
- W2034779712 hasAuthorship W2034779712A5011351573 @default.
- W2034779712 hasAuthorship W2034779712A5022455503 @default.
- W2034779712 hasAuthorship W2034779712A5050681210 @default.
- W2034779712 hasAuthorship W2034779712A5079620576 @default.
- W2034779712 hasConcept C104317684 @default.
- W2034779712 hasConcept C178790620 @default.
- W2034779712 hasConcept C179247698 @default.
- W2034779712 hasConcept C181199279 @default.
- W2034779712 hasConcept C185592680 @default.
- W2034779712 hasConcept C197404232 @default.
- W2034779712 hasConcept C206967267 @default.
- W2034779712 hasConcept C2778306172 @default.
- W2034779712 hasConcept C2778460671 @default.
- W2034779712 hasConcept C2779441561 @default.
- W2034779712 hasConcept C40767141 @default.
- W2034779712 hasConcept C43617362 @default.
- W2034779712 hasConcept C515207424 @default.
- W2034779712 hasConcept C523546767 @default.
- W2034779712 hasConcept C54355233 @default.
- W2034779712 hasConcept C55493867 @default.
- W2034779712 hasConcept C86803240 @default.
- W2034779712 hasConceptScore W2034779712C104317684 @default.
- W2034779712 hasConceptScore W2034779712C178790620 @default.
- W2034779712 hasConceptScore W2034779712C179247698 @default.
- W2034779712 hasConceptScore W2034779712C181199279 @default.
- W2034779712 hasConceptScore W2034779712C185592680 @default.
- W2034779712 hasConceptScore W2034779712C197404232 @default.
- W2034779712 hasConceptScore W2034779712C206967267 @default.
- W2034779712 hasConceptScore W2034779712C2778306172 @default.
- W2034779712 hasConceptScore W2034779712C2778460671 @default.
- W2034779712 hasConceptScore W2034779712C2779441561 @default.
- W2034779712 hasConceptScore W2034779712C40767141 @default.
- W2034779712 hasConceptScore W2034779712C43617362 @default.
- W2034779712 hasConceptScore W2034779712C515207424 @default.
- W2034779712 hasConceptScore W2034779712C523546767 @default.
- W2034779712 hasConceptScore W2034779712C54355233 @default.
- W2034779712 hasConceptScore W2034779712C55493867 @default.
- W2034779712 hasConceptScore W2034779712C86803240 @default.
- W2034779712 hasIssue "2" @default.
- W2034779712 hasLocation W20347797121 @default.
- W2034779712 hasLocation W20347797122 @default.
- W2034779712 hasOpenAccess W2034779712 @default.
- W2034779712 hasPrimaryLocation W20347797121 @default.
- W2034779712 hasRelatedWork W1998301492 @default.
- W2034779712 hasRelatedWork W2011209568 @default.
- W2034779712 hasRelatedWork W2030269044 @default.
- W2034779712 hasRelatedWork W2034779712 @default.
- W2034779712 hasRelatedWork W2070543174 @default.
- W2034779712 hasRelatedWork W2080001810 @default.
- W2034779712 hasRelatedWork W2108560269 @default.
- W2034779712 hasRelatedWork W2157714626 @default.
- W2034779712 hasRelatedWork W2257470407 @default.
- W2034779712 hasRelatedWork W2391173190 @default.
- W2034779712 hasVolume "921" @default.
- W2034779712 isParatext "false" @default.
- W2034779712 isRetracted "false" @default.
- W2034779712 magId "2034779712" @default.
- W2034779712 workType "article" @default.