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- W2034781286 abstract "During the development of the liquid chromatography with electrospray ionization-tandem mass spectrometry for the quantitative determination of 3'-C-ethynylcytidine (I) in rat plasma, ion suppression caused by the matrix components was observed for I and its structural analogue, 3'-C-ethylcytidine (II) as the internal standard. In the method initially designed, I/II peak area ratios varied according to the degree of matrix effect, which led to the poor precision of the assay. From the examination of the ion suppression behavior for I and II, it was assumed that this phenomenon is attributed to the difference in the retention time between I and II. Based on this assumption, therefore, the methanol content in the mobile phase was changed from 5 to 25% so as to make I and II the same retention time. As a result of this modification of the initial method, the precision expressed as relative standard deviation was improved from 5.2-16.2 to 2.7-4.2% in intra-assay and from 6.8-14.9 to 3.5-7.2% in inter-assay validations." @default.
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- W2034781286 date "2001-04-01" @default.
- W2034781286 modified "2023-10-16" @default.
- W2034781286 title "Improvement in precision of the liquid chromatographic–electrospray ionization tandem mass spectrometric analysis of 3′-C-ethynylcytidine in rat plasma" @default.
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- W2034781286 doi "https://doi.org/10.1016/s0378-4347(00)00588-0" @default.
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