FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034782253> ?p ?o ?g. }

• W2034782253 endingPage "508" @default.
• W2034782253 startingPage "499" @default.
• W2034782253 abstract "Parafollicular cells (PC) of the sheep thyroid gland are neural crest derivatives that synthesize and release the biogenic amine serotonin (5-HT) as well as the hormone calcitonin. The thyroid also contains a highly specific serotonin-binding protein (SBP). Separation of dissociated thyroid cells was done to study the cellular localization of SBP and to develop a means of isolating PC for study. Various methods were used to obtain an enriched and purified population of PC. Minced thyroid glands were enzymatically dissociated and the cells were layered on a Ficoll linear density gradient. Fractions obtained from the gradient were examined for cell number, viability, 5-HT concentration, SBP activity, and morphology by electron microscopy. One of the fractions was found to be enriched in PC. High levels of 5-HT and SBP were also found in this fraction, whereas these levels were low where the majority of cells were found. This PC-rich fraction, however, contained numerous follicular cells (FC); therefore, additional approaches to cell separation were used. FC can be stimulated in vitro with thyroid stimulating hormone (TSH) to become intensely phagocytic. When stimulated cells were incubated in the presence of silica microspheres, the FC engulfed the microspheres, which were toxic to them. PC did not become phagocytic and were unharmed by the microspheres. Suspended cells, after incubation with microspheres, were centrifuged on a discontinuous gradient, and a PC-rich fraction was obtained. Silica, however, interfered with analysis of SBP. Another method to take advantage of the phagocytic potential of FC was therefore used. TSH-stimulated cell suspensions were passed through a column of sepharose to which thyroglobulin had been coupled. Stimulated FC apparently adhered to the beads and were retained by the columns. Fractions eluting from the columns were greatly enriched with PC. These fractions contained high levels of 5-HT and SBP, and considerably reduced FC contamination was found by quantitative electron microscopy. It is concluded that SBP is localized to PC in the sheep thyroid. The idea that these cells resemble serotonergic neurons in their mechanisms of 5-HT storage is supported." @default.
• W2034782253 created "2016-06-24" @default.
• W2034782253 creator A5003699782 @default.
• W2034782253 creator A5008249169 @default.
• W2034782253 creator A5030863218 @default.
• W2034782253 creator A5051404866 @default.
• W2034782253 date "1981-03-01" @default.
• W2034782253 modified "2023-09-27" @default.
• W2034782253 title "Separation of dissociated thyroid follicular and parfollicular cells: association of serotonin binding protein with parafollicular cells" @default.
• W2034782253 cites W1964680205 @default.
• W2034782253 cites W1969347482 @default.
• W2034782253 cites W1970434202 @default.
• W2034782253 cites W1973981522 @default.
• W2034782253 cites W1977913687 @default.
• W2034782253 cites W1982607518 @default.
• W2034782253 cites W1999149195 @default.
• W2034782253 cites W1999324186 @default.
• W2034782253 cites W2008810880 @default.
• W2034782253 cites W2009639419 @default.
• W2034782253 cites W2012309837 @default.
• W2034782253 cites W2025513810 @default.
• W2034782253 cites W2036814823 @default.
• W2034782253 cites W2045040694 @default.
• W2034782253 cites W2048861694 @default.
• W2034782253 cites W2049158155 @default.
• W2034782253 cites W2050275542 @default.
• W2034782253 cites W2056898658 @default.
• W2034782253 cites W2057838125 @default.
• W2034782253 cites W2057876636 @default.
• W2034782253 cites W2059064047 @default.
• W2034782253 cites W2071508854 @default.
• W2034782253 cites W2073447884 @default.
• W2034782253 cites W2089025759 @default.
• W2034782253 cites W2098906642 @default.
• W2034782253 cites W2115693015 @default.
• W2034782253 cites W2127346038 @default.
• W2034782253 cites W2255326596 @default.
• W2034782253 cites W2325029590 @default.
• W2034782253 cites W2401612105 @default.
• W2034782253 cites W2417754127 @default.
• W2034782253 doi "https://doi.org/10.1083/jcb.88.3.499" @default.
• W2034782253 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2112761" @default.
• W2034782253 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7217200" @default.
• W2034782253 hasPublicationYear "1981" @default.
• W2034782253 type Work @default.
• W2034782253 sameAs 2034782253 @default.
• W2034782253 citedByCount "31" @default.
• W2034782253 countsByYear W20347822532012 @default.
• W2034782253 countsByYear W20347822532013 @default.
• W2034782253 countsByYear W20347822532017 @default.
• W2034782253 countsByYear W20347822532023 @default.
• W2034782253 crossrefType "journal-article" @default.
• W2034782253 hasAuthorship W2034782253A5003699782 @default.
• W2034782253 hasAuthorship W2034782253A5008249169 @default.
• W2034782253 hasAuthorship W2034782253A5030863218 @default.
• W2034782253 hasAuthorship W2034782253A5051404866 @default.
• W2034782253 hasBestOaLocation W20347822531 @default.
• W2034782253 hasConcept C126322002 @default.
• W2034782253 hasConcept C134018914 @default.
• W2034782253 hasConcept C137061746 @default.
• W2034782253 hasConcept C153911025 @default.
• W2034782253 hasConcept C170493617 @default.
• W2034782253 hasConcept C202751555 @default.
• W2034782253 hasConcept C25642318 @default.
• W2034782253 hasConcept C2775864247 @default.
• W2034782253 hasConcept C2776986829 @default.
• W2034782253 hasConcept C2778057883 @default.
• W2034782253 hasConcept C2779627488 @default.
• W2034782253 hasConcept C2908647359 @default.
• W2034782253 hasConcept C49039625 @default.
• W2034782253 hasConcept C526584372 @default.
• W2034782253 hasConcept C5362183 @default.
• W2034782253 hasConcept C55493867 @default.
• W2034782253 hasConcept C71924100 @default.
• W2034782253 hasConcept C86803240 @default.
• W2034782253 hasConcept C99454951 @default.
• W2034782253 hasConceptScore W2034782253C126322002 @default.
• W2034782253 hasConceptScore W2034782253C134018914 @default.
• W2034782253 hasConceptScore W2034782253C137061746 @default.
• W2034782253 hasConceptScore W2034782253C153911025 @default.
• W2034782253 hasConceptScore W2034782253C170493617 @default.
• W2034782253 hasConceptScore W2034782253C202751555 @default.
• W2034782253 hasConceptScore W2034782253C25642318 @default.
• W2034782253 hasConceptScore W2034782253C2775864247 @default.
• W2034782253 hasConceptScore W2034782253C2776986829 @default.
• W2034782253 hasConceptScore W2034782253C2778057883 @default.
• W2034782253 hasConceptScore W2034782253C2779627488 @default.
• W2034782253 hasConceptScore W2034782253C2908647359 @default.
• W2034782253 hasConceptScore W2034782253C49039625 @default.
• W2034782253 hasConceptScore W2034782253C526584372 @default.
• W2034782253 hasConceptScore W2034782253C5362183 @default.
• W2034782253 hasConceptScore W2034782253C55493867 @default.
• W2034782253 hasConceptScore W2034782253C71924100 @default.
• W2034782253 hasConceptScore W2034782253C86803240 @default.
• W2034782253 hasConceptScore W2034782253C99454951 @default.
• W2034782253 hasIssue "3" @default.
• W2034782253 hasLocation W20347822531 @default.
• W2034782253 hasLocation W20347822532 @default.

• next