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- W2034785945 abstract "DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 45:109-114 (2001) - doi:10.3354/dao045109 Comparison of single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods for detection of Myxobolus cerebralis George J. Schisler1,*, Eric P. Bergersen1, Peter G. Walker2, John Wood3, Janet K. Epp3 1Colorado Cooperative Fish and Wildlife Research Unit, 201 Wagar Building, Colorado State University, Fort Collins, Colorado 80526, USA 2Colorado Division of Wildlife, Colorado Aquatic Animal Health Lab, PO Box 128 Brush, Colorado 80723, USA 3Pisces Molecular LLC., 5311 Western Avenue Suite E, Boulder, Colorado 80301, USA *E-mail: george.schisler@state.co.us ABSTRACT: Single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods were compared for the detection of Myxobolus cerebralis. Parallel tests were conducted on a total of 1743 free-ranging and 400 hatchery-reared salmonids. Concurrent results were found in 84.6% of the free-ranging fish samples, and 83.5% of the hatchery samples. PCR identified M. cerebralis more frequently than did PTD, and did so in many geographic locations previously considered free of the parasite. Average myxospore count by PTD among both free-ranging and hatchery fish increased significantly (p < 0.001) with a subjective evaluation of amplicon staining intensity. KEY WORDS: Whirling disease · Myxobolus cerebralis · Pepsin-trypsin digest (PTD) · Polymerase chain reaction (PCR) · Testing methods Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 45, No. 2. Online publication date: June 20, 2001 Print ISSN: 0177-5103; Online ISSN: 1616-1580 Copyright © 2001 Inter-Research." @default.
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- W2034785945 title "Comparison of single-round polymerase chain reaction (PCR) and pepsin-trypsin digest (PTD) methods for detection of Myxobolus cerebralis" @default.
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- W2034785945 doi "https://doi.org/10.3354/dao045109" @default.
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