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• W2034787548 abstract "ABSTRACT The P143 protein of Autographa californica nuclear polyhedrosis virus is essential for replication of viral DNA. To determine the function of P143, the protein was purified to near homogeneity from recombinant baculovirus-infected cells that overexpress P143. ATPase activity copurified with P143 protein during purification and also during gel filtration at a high salt concentration. The ATPase activity did not require the presence of single-stranded DNA, but was stimulated fourfold by the addition of single-stranded DNA. The ATPase activity of P143 had a K m of 60 μM and a turnover of 4.5 molecules of ATP hydrolyzed/s/molecule of enzyme, indicating moderate affinity for ATP and high catalytic efficiency. P143 unwound a 40-nucleotide primer in an ATP-dependent manner, indicating that the enzyme possesses in vitro DNA helicase activity. Based on this result, it seems likely that P143 functions as a helicase in viral DNA replication." @default.
• W2034787548 created "2016-06-24" @default.
• W2034787548 creator A5061013999 @default.
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• W2034787548 date "2000-06-01" @default.
• W2034787548 modified "2023-10-17" @default.
• W2034787548 title "The <i>Autographa californica</i> Nuclear Polyhedrosis Virus <i>p143</i> Gene Encodes a DNA Helicase" @default.
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• W2034787548 doi "https://doi.org/10.1128/jvi.74.11.5273-5279.2000" @default.
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