Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034793790> ?p ?o ?g. }
- W2034793790 abstract "U1 snRNP binds to the 5' exon-intron junction of pre-mRNA and thus plays a crucial role at an early stage of pre-mRNA splicing. We present two crystal structures of engineered U1 sub-structures, which together reveal at atomic resolution an almost complete network of protein-protein and RNA-protein interactions within U1 snRNP, and show how the 5' splice site of pre-mRNA is recognised by U1 snRNP. The zinc-finger of U1-C interacts with the duplex between pre-mRNA and the 5'-end of U1 snRNA. The binding of the RNA duplex is stabilized by hydrogen bonds and electrostatic interactions between U1-C and the RNA backbone around the splice junction but U1-C makes no base-specific contacts with pre-mRNA. The structure, together with RNA binding assays, shows that the selection of 5'-splice site nucleotides by U1 snRNP is achieved predominantly through basepairing with U1 snRNA whilst U1-C fine-tunes relative affinities of mismatched 5'-splice sites." @default.
- W2034793790 created "2016-06-24" @default.
- W2034793790 creator A5056271884 @default.
- W2034793790 creator A5068936914 @default.
- W2034793790 creator A5070955667 @default.
- W2034793790 creator A5084843841 @default.
- W2034793790 date "2015-01-02" @default.
- W2034793790 modified "2023-10-18" @default.
- W2034793790 title "Crystal structure of human U1 snRNP, a small nuclear ribonucleoprotein particle, reveals the mechanism of 5′ splice site recognition" @default.
- W2034793790 cites W131186105 @default.
- W2034793790 cites W1480821243 @default.
- W2034793790 cites W1512001995 @default.
- W2034793790 cites W1530522395 @default.
- W2034793790 cites W1553769217 @default.
- W2034793790 cites W1555757452 @default.
- W2034793790 cites W177901988 @default.
- W2034793790 cites W1873199501 @default.
- W2034793790 cites W1914793851 @default.
- W2034793790 cites W1968843636 @default.
- W2034793790 cites W1969813445 @default.
- W2034793790 cites W1979455742 @default.
- W2034793790 cites W1979765627 @default.
- W2034793790 cites W1979907792 @default.
- W2034793790 cites W1982610079 @default.
- W2034793790 cites W1988455121 @default.
- W2034793790 cites W1993926172 @default.
- W2034793790 cites W2001641653 @default.
- W2034793790 cites W2001732976 @default.
- W2034793790 cites W2003016053 @default.
- W2034793790 cites W2007134218 @default.
- W2034793790 cites W2016391746 @default.
- W2034793790 cites W2021506799 @default.
- W2034793790 cites W2022346667 @default.
- W2034793790 cites W2024170620 @default.
- W2034793790 cites W2024588724 @default.
- W2034793790 cites W2029054549 @default.
- W2034793790 cites W2029693509 @default.
- W2034793790 cites W2037356132 @default.
- W2034793790 cites W2038840577 @default.
- W2034793790 cites W2044862603 @default.
- W2034793790 cites W2051694176 @default.
- W2034793790 cites W2055644037 @default.
- W2034793790 cites W2059648068 @default.
- W2034793790 cites W2061306932 @default.
- W2034793790 cites W2063206231 @default.
- W2034793790 cites W2074302728 @default.
- W2034793790 cites W2075120021 @default.
- W2034793790 cites W2078819000 @default.
- W2034793790 cites W2086799261 @default.
- W2034793790 cites W2090121198 @default.
- W2034793790 cites W2095249676 @default.
- W2034793790 cites W2103999254 @default.
- W2034793790 cites W2106967677 @default.
- W2034793790 cites W2107787782 @default.
- W2034793790 cites W2111482169 @default.
- W2034793790 cites W2113738713 @default.
- W2034793790 cites W2113974046 @default.
- W2034793790 cites W2114520383 @default.
- W2034793790 cites W2115729502 @default.
- W2034793790 cites W2115948084 @default.
- W2034793790 cites W2124026197 @default.
- W2034793790 cites W2126846097 @default.
- W2034793790 cites W2133199944 @default.
- W2034793790 cites W2138148973 @default.
- W2034793790 cites W2139312181 @default.
- W2034793790 cites W2148393113 @default.
- W2034793790 cites W2148768032 @default.
- W2034793790 cites W2159328527 @default.
- W2034793790 cites W2163341755 @default.
- W2034793790 cites W2164983569 @default.
- W2034793790 cites W2165739214 @default.
- W2034793790 cites W2166706771 @default.
- W2034793790 cites W2169610660 @default.
- W2034793790 cites W2169669582 @default.
- W2034793790 cites W2181723092 @default.
- W2034793790 cites W92004496 @default.
- W2034793790 doi "https://doi.org/10.7554/elife.04986" @default.
- W2034793790 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4383343" @default.
- W2034793790 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25555158" @default.
- W2034793790 hasPublicationYear "2015" @default.
- W2034793790 type Work @default.
- W2034793790 sameAs 2034793790 @default.
- W2034793790 citedByCount "194" @default.
- W2034793790 countsByYear W20347937902015 @default.
- W2034793790 countsByYear W20347937902016 @default.
- W2034793790 countsByYear W20347937902017 @default.
- W2034793790 countsByYear W20347937902018 @default.
- W2034793790 countsByYear W20347937902019 @default.
- W2034793790 countsByYear W20347937902020 @default.
- W2034793790 countsByYear W20347937902021 @default.
- W2034793790 countsByYear W20347937902022 @default.
- W2034793790 countsByYear W20347937902023 @default.
- W2034793790 crossrefType "journal-article" @default.
- W2034793790 hasAuthorship W2034793790A5056271884 @default.
- W2034793790 hasAuthorship W2034793790A5068936914 @default.
- W2034793790 hasAuthorship W2034793790A5070955667 @default.
- W2034793790 hasAuthorship W2034793790A5084843841 @default.
- W2034793790 hasBestOaLocation W20347937901 @default.
- W2034793790 hasConcept C100299639 @default.
- W2034793790 hasConcept C104317684 @default.