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- W2034795288 abstract "Thiolester linked lipids in rat apolipoprotein B (ApoB) were examined by incubating reduced and carboxymethylated ApoB in 6 M urea buffer with [14C]methylamine at pH 8.5, 30°C. It was observed that [14C]methylamine was covalently incorporated into ApoB, and there was a [14C]methylamine modified product which was lipid in nature. After extraction with organic solvents, the [14C]methylamine labeled product showed its Rf on TLC to be similar to that of the synthetic N-methyl fatty acyl amide. After purification on TLC and transesterification with 3 N methanolic HCl, methyl esters of C16:0, C18:0 and C18:1 fatty acids were identified by gas-liquid chromatography. These results suggest that rat ApoB, similar to human ApoB, contained covalently linked fatty acids through the high energy, labile thiolester bonds." @default.
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- W2034795288 date "1989-08-01" @default.
- W2034795288 modified "2023-09-26" @default.
- W2034795288 title "Presence of covalently attached fatty acids in rat apolipoprotein B via thiolester linkages" @default.
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- W2034795288 doi "https://doi.org/10.1016/0006-291x(89)90845-0" @default.
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