Matches in SemOpenAlex for { <https://semopenalex.org/work/W203479682> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W203479682 abstract "Myocardial regeneration can occur in humans, albeit with very low efficiency. To improve regeneration of the injured myocardium, one can enhance the intrinsic capacity of the heart to regenerate itself and/or replace the damaged tissue by cell transplantation. Recently, we have isolated cardiomyocyte progenitor cells (CMPCs) from human fetal hearts and from adult human biopsies that can be expanded in culture and efficiently differentiated into beating cardiomyocytes. In addition, these CMPCs also efficiently differentiate into tubular structures on matrigel, forming aligned endothelial cells surrounded by smooth muscle cells. Recently, several studies have demonstrated that microRNAs (miRNAs) are important for transcriptional regulation during angiogenesis, heart development, and maintenance of stem cell populations by translational repression. We hypothesize that, since miRNAs regulate stem cell maintenance, miRNAs are involved in proliferation/differentiation of the human cardiomyocytes progenitor cells in vitro . Methods and results: Human fetal CMPCs were isolated, cultured and efficiently differentiated into beating cardiomyocytes. We observed that the muscle-specific miRNA-1 was highly upregulated (qRT-PCR, >7000 fold) in differentiated cardiomyocytes and hardly present in proliferating CMPCs. Transient transfection of miRNA-1 in undifferentiated CMPCs reduced their proliferation rate by 25% (MTT assay and FACS analysis) and enhanced differentiation into cardiomyocytes. When undifferentiated CMPCs were transiently transfected with miRNA-1 and seeded onto matrigel, angiogenic morphology was changed. The mean length and size of the tubules was significantly increased (2.5 and 4.5 fold, respectively), whereas the number of junctions was reduced by 50%. This suggests that more CMPCs were directed into the smooth muscle-like phenotype and not into branching endothelial cells, leading to larger tubules with less side junctions. Conclusion: Our results demonstrate a strong regulatory role of miRNAs in human CMPC proliferation and differentiation into cardiomyocytes. miRNA-1 functions as a key regulator, and is involved in the onset of human CMPC differentiation into cardiomyocytes and smooth muscle like cells." @default.
- W203479682 created "2016-06-24" @default.
- W203479682 creator A5000838930 @default.
- W203479682 creator A5005834517 @default.
- W203479682 creator A5027749833 @default.
- W203479682 creator A5059001298 @default.
- W203479682 creator A5081338491 @default.
- W203479682 creator A5082040556 @default.
- W203479682 date "2007-10-16" @default.
- W203479682 modified "2023-09-26" @default.
- W203479682 title "Abstract 1110: MicroRNA-1 Regulates Cardiomyocyte Differentiation And Proliferation In Human-derived Cardiomyocyte Progenitor Cells." @default.
- W203479682 hasPublicationYear "2007" @default.
- W203479682 type Work @default.
- W203479682 sameAs 203479682 @default.
- W203479682 citedByCount "0" @default.
- W203479682 crossrefType "journal-article" @default.
- W203479682 hasAuthorship W203479682A5000838930 @default.
- W203479682 hasAuthorship W203479682A5005834517 @default.
- W203479682 hasAuthorship W203479682A5027749833 @default.
- W203479682 hasAuthorship W203479682A5059001298 @default.
- W203479682 hasAuthorship W203479682A5081338491 @default.
- W203479682 hasAuthorship W203479682A5082040556 @default.
- W203479682 hasConcept C104317684 @default.
- W203479682 hasConcept C123012128 @default.
- W203479682 hasConcept C145059251 @default.
- W203479682 hasConcept C148738053 @default.
- W203479682 hasConcept C171056886 @default.
- W203479682 hasConcept C201750760 @default.
- W203479682 hasConcept C202751555 @default.
- W203479682 hasConcept C207200792 @default.
- W203479682 hasConcept C2778608917 @default.
- W203479682 hasConcept C2780394083 @default.
- W203479682 hasConcept C28328180 @default.
- W203479682 hasConcept C502942594 @default.
- W203479682 hasConcept C54009773 @default.
- W203479682 hasConcept C54355233 @default.
- W203479682 hasConcept C62112901 @default.
- W203479682 hasConcept C71924100 @default.
- W203479682 hasConcept C81885089 @default.
- W203479682 hasConcept C86803240 @default.
- W203479682 hasConcept C95444343 @default.
- W203479682 hasConceptScore W203479682C104317684 @default.
- W203479682 hasConceptScore W203479682C123012128 @default.
- W203479682 hasConceptScore W203479682C145059251 @default.
- W203479682 hasConceptScore W203479682C148738053 @default.
- W203479682 hasConceptScore W203479682C171056886 @default.
- W203479682 hasConceptScore W203479682C201750760 @default.
- W203479682 hasConceptScore W203479682C202751555 @default.
- W203479682 hasConceptScore W203479682C207200792 @default.
- W203479682 hasConceptScore W203479682C2778608917 @default.
- W203479682 hasConceptScore W203479682C2780394083 @default.
- W203479682 hasConceptScore W203479682C28328180 @default.
- W203479682 hasConceptScore W203479682C502942594 @default.
- W203479682 hasConceptScore W203479682C54009773 @default.
- W203479682 hasConceptScore W203479682C54355233 @default.
- W203479682 hasConceptScore W203479682C62112901 @default.
- W203479682 hasConceptScore W203479682C71924100 @default.
- W203479682 hasConceptScore W203479682C81885089 @default.
- W203479682 hasConceptScore W203479682C86803240 @default.
- W203479682 hasConceptScore W203479682C95444343 @default.
- W203479682 hasLocation W2034796821 @default.
- W203479682 hasOpenAccess W203479682 @default.
- W203479682 hasPrimaryLocation W2034796821 @default.
- W203479682 hasRelatedWork W1977015206 @default.
- W203479682 hasRelatedWork W1979608640 @default.
- W203479682 hasRelatedWork W1985100709 @default.
- W203479682 hasRelatedWork W2001356111 @default.
- W203479682 hasRelatedWork W2017587374 @default.
- W203479682 hasRelatedWork W2035022579 @default.
- W203479682 hasRelatedWork W2154896283 @default.
- W203479682 hasRelatedWork W2264413950 @default.
- W203479682 hasRelatedWork W2316316092 @default.
- W203479682 hasRelatedWork W2335134891 @default.
- W203479682 hasRelatedWork W2352263242 @default.
- W203479682 hasRelatedWork W2374589300 @default.
- W203479682 hasRelatedWork W2743087301 @default.
- W203479682 hasRelatedWork W2796297947 @default.
- W203479682 hasRelatedWork W2991590985 @default.
- W203479682 hasRelatedWork W3007564483 @default.
- W203479682 hasRelatedWork W3153663979 @default.
- W203479682 hasRelatedWork W3209227569 @default.
- W203479682 hasRelatedWork W1520444026 @default.
- W203479682 hasRelatedWork W2182359936 @default.
- W203479682 hasVolume "116" @default.
- W203479682 isParatext "false" @default.
- W203479682 isRetracted "false" @default.
- W203479682 magId "203479682" @default.
- W203479682 workType "article" @default.