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• W2034800617 startingPage "1282" @default.
• W2034800617 abstract "We previously demonstrated that transforming growth factor-beta1 (TGF-beta1), while having no effect alone, enhances nitric oxide (NO) production in primary, purified mouse astrocytes induced by lipopolysaccharide (LPS) plus interferon-gamma (IFN-gamma), by recruiting a latent population of astrocytes to respond, thereby enhancing the total number of cells that express Nos2. In this investigation, we evaluated the molecular signaling pathway by which this occurs. We found that purified murine primary astrocytes express mRNA for TGFbetaRII as well as the TGFbetaRI subunit activin-like kinase 5 (ALK5), but not ALK1. Immunofluorescence microscopy confirmed the expression of TGFbetaRII and ALK5 protein in astrocytes. Consistent with ALK5 signaling, Smad3 accumulated in the nucleus of astrocytes as early as 30 min after TGF-beta1 (3 ng/mL) treatment and persisted upto 32 hr after TGF-beta1 administration. Addition of ALK5 inhibitors prevented TGF-beta1-mediated Smad3 nuclear accumulation and NO production when given prior to the Nos2 induction stimuli, but not after. Finally, astrocyte cultures derived from Smad3 null mutant mice did not exhibit a TGF-beta1-mediated increase in iNOS expression. Overall, this data suggests that ALK5 signaling and Smad3 nuclear accumulation is required for optimal enhancement of LPS plus IFNgamma-induced NO production in astrocytes by TGF-beta1." @default.
• W2034800617 created "2016-06-24" @default.
• W2034800617 creator A5009669991 @default.
• W2034800617 creator A5012317445 @default.
• W2034800617 creator A5026002747 @default.
• W2034800617 date "2010-05-05" @default.
• W2034800617 modified "2023-10-17" @default.
• W2034800617 title "Smad3-dependent signaling underlies the TGF-β1-mediated enhancement in astrocytic iNOS expression" @default.
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• W2034800617 doi "https://doi.org/10.1002/glia.21005" @default.
• W2034800617 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4451602" @default.
• W2034800617 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20607716" @default.
• W2034800617 hasPublicationYear "2010" @default.
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