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- W2034809305 abstract "Salmon testis is a good source of DNA nicking–closing (N–C) enzyme, as expected for rapidly proliferating cells. Partial purification was obtained but it resulted in an unstable form of N–C enzyme. Cruder fractions are useful for relaxing supercoiled DNA under conditions where other N–C enzymes are inactive. The enzyme is very tolerant of salt concentration, with activity still detectable at 0.6 M NaCl. It is also active at low temperatures with reasonable activity at 0 °C. However, at temperatures greater than 25 °C it is rapidly inactivated. Otherwise, its properties are similar to other eucaryotic N–C enzymes." @default.
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- W2034809305 date "1978-02-01" @default.
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- W2034809305 title "DNA nicking–closing activity from salmon testis" @default.
- W2034809305 doi "https://doi.org/10.1139/o78-014" @default.
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