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- W2034844564 abstract "Abstract This paper presents a method to observe the motions and configurations of large DNA molecules undergoing capillary electrophoresis (CE). A simple device to perform CE horizontally under microscopic observation is designed and images of single DNA molecules inside the capillary are obtained using an epi-fluorescence microscope. DNA molecules moved towards the negative electrode when an electric field was applied. The mobilities of three types of DNA (T4 and χ bacteriophage DNA and PBR322 plasmid DNA) were measured at different electric field strength. The mobility vs. electric field strength curves of these three large DNAs showed that the mobility remained constant at high electric field strength (200–600 Volt/cm) and increased significantly at low electric field strength (⩽ 50 Volt/cm.). The apparent mobilities of the large DNA molecules were independent of molecular weight. At electric field strengths ⩾ 400 Volt/cm., big aggregates (snowballs) of DNA molecules formed and moved upstream towards the positive electrode. When the field was turned off, the aggregates dissociated into a cloud of single DNA molecules, and diffused into the solution." @default.
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- W2034844564 date "1991-12-01" @default.
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- W2034844564 title "Observation of DNA Molecules Undergoing Capillary Electrophoresis" @default.
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- W2034844564 doi "https://doi.org/10.1080/07391102.1991.10507934" @default.
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