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• W2034854962 abstract "Vesicular exocytosis plays an important role in many physiological processes. The dense-core vesicles release of chromaffin cells is a suitable model for the presynaptic process in neurosecretory cells. In this study, light-addressable potentiometric sensor (LAPS) was introduced as a label-free recording method for vesicle release by the local extracellular acidification. The chromaffin cells are directly cultured on the sensor surface. After cells and LAPS hybrid system is established, the events of vesicular exocytosis are recorded. Protons stored in the vesicles and co-released with transmitters, induced a brief acidic shifts in the cell-sensor cleft. Under the stimulation of the KCl and acetylcholine (Ach), the signals presented the different amplitude and exocytosis rate, and reflected the specific features of the exocytosis. The result indicates that neurosecretory cell-based biosensor will provide a useful platform for neurosecretion mechanism research by monitoring the exocytotic activities with extracellular acidification sensing." @default.
• W2034854962 created "2016-06-24" @default.
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• W2034854962 date "2012-05-01" @default.
• W2034854962 modified "2023-10-17" @default.
• W2034854962 title "Neurosecretory cell-based biosensor: Monitoring secretion of adrenal chromaffin cells by local extracellular acidification using light-addressable potentiometric sensor" @default.
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• W2034854962 doi "https://doi.org/10.1016/j.bios.2012.02.022" @default.
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