FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034871552> ?p ?o ?g. }

• W2034871552 endingPage "708" @default.
• W2034871552 startingPage "699" @default.
• W2034871552 abstract "Ethanol intake depletes the mitochondrial pool of reduced glutathione (GSH) by impairing the transport of GSH from cytosol into mitochondria. S-Adenosyl-L-methionine (SAM) supplementation of ethanol-fed rats restores the mitochondrial pool of GSH. The purpose of the current study was to determine the effect of ethanol feeding on the kinetic parameters of mitochondrial GSH transport, the fluidity of mitochondria, and the effect of SAM on these changes. Male Sprague-Dawley rats were fed ethanol-liquid diet for 4 weeks supplemented with either SAM or N-acetylcysteine (NAC). SAM-supplementation of ethanol-fed rats restored the mitochondrial GSH pool but NAC administration did not. Kinetic studies of GSH transport in isolated mitochondria revealed two saturable, adenosine triphosphate (ATP)-stimulated components that were affected significantly by chronic ethanol feeding: Lowering Vmax (0.22 and 1.6 in ethanol case vs. 0.44 and 2.7 nmol/15 sec/mg protein in controls) for both low and high affinity components with the latter showing an increased Km (15.5 vs. 8.9, mmol/L in ethanol vs. control). Mitochondria from SAM-supplemented ethanol-fed rats showed kinetic features of GSH transport similar to control mitochondria. Determination of membrane fluidity revealed an increased order parameter in ethanol compared with control mitochondria, which was restricted to the polar head groups of the bilayer and was prevented by SAM but not NAC supplementation of ethanol-fed rats. The changes elicited in mitochondria by ethanol were confined to the inner membrane; mitoplasts from ethanol-fed rats showed features similar to those of intact mitochondria such as impaired transport of GSH and increased order parameter. A different mitochondrial transporter, adenosine diphosphate (ADP)/ATP translocator, was unaffected by ethanol feeding. Furthermore, fluidization of mitochondria or mitoplasts from ethanol-fed rats by treatment with a fatty acid derivative restored their ability to transport GSH to control levels. Thus, ethanol-induced impaired transport of GSH into mitochondria is selective, mediated by decreased fluidity of the mitochondrial inner membrane, and prevented by SAM treatment." @default.
• W2034871552 created "2016-06-24" @default.
• W2034871552 creator A5029888033 @default.
• W2034871552 creator A5043356624 @default.
• W2034871552 creator A5048561028 @default.
• W2034871552 creator A5058717462 @default.
• W2034871552 creator A5061842402 @default.
• W2034871552 creator A5074070131 @default.
• W2034871552 creator A5080139466 @default.
• W2034871552 creator A5080875810 @default.
• W2034871552 date "1997-09-01" @default.
• W2034871552 modified "2023-10-18" @default.
• W2034871552 title "Transport of reduced glutathione in hepatic mitochondria and mitoplasts from ethanol-treated rats: Effect of membrane physical properties andS-adenosyl-L-methionine" @default.
• W2034871552 cites W1431301684 @default.
• W2034871552 cites W1509034405 @default.
• W2034871552 cites W1514411721 @default.
• W2034871552 cites W1557322059 @default.
• W2034871552 cites W1562743220 @default.
• W2034871552 cites W159390204 @default.
• W2034871552 cites W1944988970 @default.
• W2034871552 cites W1950610522 @default.
• W2034871552 cites W1964047654 @default.
• W2034871552 cites W1966461945 @default.
• W2034871552 cites W1973363737 @default.
• W2034871552 cites W1974169493 @default.
• W2034871552 cites W1974794685 @default.
• W2034871552 cites W1980387958 @default.
• W2034871552 cites W1983788916 @default.
• W2034871552 cites W1997243937 @default.
• W2034871552 cites W2005859045 @default.
• W2034871552 cites W2012185275 @default.
• W2034871552 cites W2018567941 @default.
• W2034871552 cites W2024659032 @default.
• W2034871552 cites W2026496585 @default.
• W2034871552 cites W2031684858 @default.
• W2034871552 cites W2032872396 @default.
• W2034871552 cites W2043329777 @default.
• W2034871552 cites W2043335917 @default.
• W2034871552 cites W2046985709 @default.
• W2034871552 cites W2058284453 @default.
• W2034871552 cites W2075697830 @default.
• W2034871552 cites W2075902854 @default.
• W2034871552 cites W2076876812 @default.
• W2034871552 cites W2087201094 @default.
• W2034871552 cites W2087598382 @default.
• W2034871552 cites W2092898235 @default.
• W2034871552 cites W2096271036 @default.
• W2034871552 cites W2104010651 @default.
• W2034871552 cites W2125562918 @default.
• W2034871552 cites W2155216573 @default.
• W2034871552 cites W2168526937 @default.
• W2034871552 cites W2181148302 @default.
• W2034871552 cites W2275889189 @default.
• W2034871552 cites W2401130413 @default.
• W2034871552 cites W2401153079 @default.
• W2034871552 cites W2426267741 @default.
• W2034871552 cites W49713821 @default.
• W2034871552 doi "https://doi.org/10.1002/hep.510260323" @default.
• W2034871552 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9303501" @default.
• W2034871552 hasPublicationYear "1997" @default.
• W2034871552 type Work @default.
• W2034871552 sameAs 2034871552 @default.
• W2034871552 citedByCount "51" @default.
• W2034871552 countsByYear W20348715522012 @default.
• W2034871552 countsByYear W20348715522013 @default.
• W2034871552 countsByYear W20348715522014 @default.
• W2034871552 countsByYear W20348715522015 @default.
• W2034871552 countsByYear W20348715522016 @default.
• W2034871552 countsByYear W20348715522019 @default.
• W2034871552 countsByYear W20348715522021 @default.
• W2034871552 countsByYear W20348715522022 @default.
• W2034871552 countsByYear W20348715522023 @default.
• W2034871552 crossrefType "journal-article" @default.
• W2034871552 hasAuthorship W2034871552A5029888033 @default.
• W2034871552 hasAuthorship W2034871552A5043356624 @default.
• W2034871552 hasAuthorship W2034871552A5048561028 @default.
• W2034871552 hasAuthorship W2034871552A5058717462 @default.
• W2034871552 hasAuthorship W2034871552A5061842402 @default.
• W2034871552 hasAuthorship W2034871552A5074070131 @default.
• W2034871552 hasAuthorship W2034871552A5080139466 @default.
• W2034871552 hasAuthorship W2034871552A5080875810 @default.
• W2034871552 hasConcept C126322002 @default.
• W2034871552 hasConcept C181199279 @default.
• W2034871552 hasConcept C185592680 @default.
• W2034871552 hasConcept C2778918659 @default.
• W2034871552 hasConcept C2779564974 @default.
• W2034871552 hasConcept C2780161600 @default.
• W2034871552 hasConcept C2780912031 @default.
• W2034871552 hasConcept C2781302388 @default.
• W2034871552 hasConcept C28859421 @default.
• W2034871552 hasConcept C41625074 @default.
• W2034871552 hasConcept C515207424 @default.
• W2034871552 hasConcept C538909803 @default.
• W2034871552 hasConcept C55493867 @default.
• W2034871552 hasConcept C71924100 @default.
• W2034871552 hasConcept C75385678 @default.
• W2034871552 hasConcept C98539663 @default.
• W2034871552 hasConceptScore W2034871552C126322002 @default.

• next