FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034874325> ?p ?o ?g. }

• W2034874325 endingPage "357" @default.
• W2034874325 startingPage "348" @default.
• W2034874325 abstract "Molecular methods for quantifying defined Bacteroidales species from the human gastrointestinal tract may have important clinical and environmental applications, ranging from diagnosis of infections to fecal source tracking in surface waters. In this study, sequences from the V2 region of the small subunit ribosomal RNA gene were targeted in the development of qPCR assays to quantify DNA from six Bacteroides and one Prevotella species. In silico and experimental analyses suggested that each of the assays was highly discriminatory in detecting DNA from the intended species. Analytical sensitivity, precision and ranges of quantification were demonstrated for each assay by coefficients of variation of less than 2% for cycle threshold measurements over a range from 10 to 4×10(4) target sequence copies. The assays were applied to assess the occurrence and relative abundance of their target sequences in feces from humans and five animal groups as well as in 14 sewage samples from 13 different treatment facilities. Sequences from each of the species were detected at high levels (>10(3)copies/ng total extracted DNA) in human wastes. Sequences were also detected by each assay in all sewage samples and, with exception of the Prevotella sequences, showed highly correlated (R(2)≥0.7) variations in concentrations between samples. In contrast, the occurrence and relative abundance profiles of these sequences differed substantially in the fecal samples from each of the animal groups. These results suggest that analyses for multiple individual Bacteroidales species may be useful in identifying human fecal pollution in environmental waters." @default.
• W2034874325 created "2016-06-24" @default.
• W2034874325 creator A5017606898 @default.
• W2034874325 creator A5027451149 @default.
• W2034874325 creator A5033446224 @default.
• W2034874325 creator A5049503724 @default.
• W2034874325 creator A5055755303 @default.
• W2034874325 creator A5090803759 @default.
• W2034874325 date "2010-10-01" @default.
• W2034874325 modified "2023-10-11" @default.
• W2034874325 title "Evaluation of genetic markers from the 16S rRNA gene V2 region for use in quantitative detection of selected Bacteroidales species and human fecal waste by qPCR" @default.
• W2034874325 cites W1480964994 @default.
• W2034874325 cites W1970699069 @default.
• W2034874325 cites W1972031592 @default.
• W2034874325 cites W1974168291 @default.
• W2034874325 cites W1978672597 @default.
• W2034874325 cites W1981675027 @default.
• W2034874325 cites W1982031096 @default.
• W2034874325 cites W1987375372 @default.
• W2034874325 cites W1992155056 @default.
• W2034874325 cites W1998601670 @default.
• W2034874325 cites W1999436404 @default.
• W2034874325 cites W2006404639 @default.
• W2034874325 cites W2006829372 @default.
• W2034874325 cites W2008644502 @default.
• W2034874325 cites W2012469769 @default.
• W2034874325 cites W2019925568 @default.
• W2034874325 cites W2020021269 @default.
• W2034874325 cites W2030027451 @default.
• W2034874325 cites W2032067970 @default.
• W2034874325 cites W2041018779 @default.
• W2034874325 cites W2041366022 @default.
• W2034874325 cites W2043988772 @default.
• W2034874325 cites W2044008717 @default.
• W2034874325 cites W2051434251 @default.
• W2034874325 cites W2058665460 @default.
• W2034874325 cites W2068592081 @default.
• W2034874325 cites W2081978855 @default.
• W2034874325 cites W2087680447 @default.
• W2034874325 cites W2095920248 @default.
• W2034874325 cites W2099478404 @default.
• W2034874325 cites W2100171490 @default.
• W2034874325 cites W2102555068 @default.
• W2034874325 cites W2105973142 @default.
• W2034874325 cites W2106882534 @default.
• W2034874325 cites W2121409138 @default.
• W2034874325 cites W2124054919 @default.
• W2034874325 cites W2137678549 @default.
• W2034874325 cites W2138540066 @default.
• W2034874325 cites W2146713330 @default.
• W2034874325 cites W2148938510 @default.
• W2034874325 cites W2150716871 @default.
• W2034874325 cites W2161996694 @default.
• W2034874325 cites W2166905439 @default.
• W2034874325 cites W4254275792 @default.
• W2034874325 doi "https://doi.org/10.1016/j.syapm.2010.06.001" @default.
• W2034874325 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20655680" @default.
• W2034874325 hasPublicationYear "2010" @default.
• W2034874325 type Work @default.
• W2034874325 sameAs 2034874325 @default.
• W2034874325 citedByCount "185" @default.
• W2034874325 countsByYear W20348743252012 @default.
• W2034874325 countsByYear W20348743252013 @default.
• W2034874325 countsByYear W20348743252014 @default.
• W2034874325 countsByYear W20348743252015 @default.
• W2034874325 countsByYear W20348743252016 @default.
• W2034874325 countsByYear W20348743252017 @default.
• W2034874325 countsByYear W20348743252018 @default.
• W2034874325 countsByYear W20348743252019 @default.
• W2034874325 countsByYear W20348743252020 @default.
• W2034874325 countsByYear W20348743252021 @default.
• W2034874325 countsByYear W20348743252022 @default.
• W2034874325 countsByYear W20348743252023 @default.
• W2034874325 crossrefType "journal-article" @default.
• W2034874325 hasAuthorship W2034874325A5017606898 @default.
• W2034874325 hasAuthorship W2034874325A5027451149 @default.
• W2034874325 hasAuthorship W2034874325A5033446224 @default.
• W2034874325 hasAuthorship W2034874325A5049503724 @default.
• W2034874325 hasAuthorship W2034874325A5055755303 @default.
• W2034874325 hasAuthorship W2034874325A5090803759 @default.
• W2034874325 hasConcept C104317684 @default.
• W2034874325 hasConcept C122325731 @default.
• W2034874325 hasConcept C127413603 @default.
• W2034874325 hasConcept C168007829 @default.
• W2034874325 hasConcept C171636592 @default.
• W2034874325 hasConcept C18903297 @default.
• W2034874325 hasConcept C2775905019 @default.
• W2034874325 hasConcept C2776357993 @default.
• W2034874325 hasConcept C2777392542 @default.
• W2034874325 hasConcept C2778168646 @default.
• W2034874325 hasConcept C2780423288 @default.
• W2034874325 hasConcept C42062724 @default.
• W2034874325 hasConcept C49105822 @default.
• W2034874325 hasConcept C523546767 @default.
• W2034874325 hasConcept C54355233 @default.
• W2034874325 hasConcept C548081761 @default.
• W2034874325 hasConcept C58790150 @default.
• W2034874325 hasConcept C61716771 @default.

• next