Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034884520> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2034884520 endingPage "76" @default.
- W2034884520 startingPage "59" @default.
- W2034884520 abstract "In a previous publication we introduced the hen’s egg test for micronucleus induction (HET-MN) as an extremely simple, inexpensive and rapid animal free genotoxicity assay which is positioned between pure in vitro and in vivo assays, strictly in line with animal protection regulations and ethical aspects. The HET-MN combines the use of the commonly accepted genetic endpoint “formation of micronuclei” with the well characterized and complex model of the chick embryo. The high metabolic competency provided by this model enables metabolic activation, elimination and excretion of xenobiotics including mutagens and promutagens. In this paper we present some new methodological aspects, which are important for improving the experimental protocol. We used cyclophosphamide (CP) and 7,12-dimethyl-benz[a]anthracene (DMBA) as model substances. Dose-response-relationship for both chemicals and cytotoxic effects for CP are described. In addition to the standard proliferation marker PCE/NCE-ratio we found an increased frequency of primitive erythrocytes (E I) and the appearance of proerythroblasts and erythroblasts as further alerting signals for cytotoxic or erythrosuppressive effects. From the total cell population we could further qualify the group of target cells. We found that all definite erythrocytes (E II), observed at day 11 (d11), are relevant target cells, independent from their stage of maturity (polychromatic as well as normochromatic definite erythrocytes). E I cells do not belong to the group of target cells, however. An additional important methodological aspect is the optimal time frame. We found the time period from d8 of incubation (administration of the test substance) up to d11 (time point of blood sampling) as most favorable. In this way an exposure period of up to 72 h is covered. Further results indicate that the air cell route provides a higher response to the test substances than the albumen route. The consideration of the described methodological aspects will contribute to the improvement of the experimental protocol of the HET-MN." @default.
- W2034884520 created "2016-06-24" @default.
- W2034884520 creator A5024040176 @default.
- W2034884520 creator A5035093379 @default.
- W2034884520 creator A5044635824 @default.
- W2034884520 date "2002-02-01" @default.
- W2034884520 modified "2023-10-10" @default.
- W2034884520 title "Some new methodological aspects of the hen’s egg test for micronucleus induction (HET-MN)" @default.
- W2034884520 cites W1990494717 @default.
- W2034884520 cites W2028922268 @default.
- W2034884520 cites W2038270678 @default.
- W2034884520 cites W2055139893 @default.
- W2034884520 cites W2059148896 @default.
- W2034884520 cites W2087061438 @default.
- W2034884520 cites W335160920 @default.
- W2034884520 cites W4211236496 @default.
- W2034884520 doi "https://doi.org/10.1016/s1383-5718(01)00317-5" @default.
- W2034884520 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11815245" @default.
- W2034884520 hasPublicationYear "2002" @default.
- W2034884520 type Work @default.
- W2034884520 sameAs 2034884520 @default.
- W2034884520 citedByCount "29" @default.
- W2034884520 countsByYear W20348845202012 @default.
- W2034884520 countsByYear W20348845202013 @default.
- W2034884520 countsByYear W20348845202014 @default.
- W2034884520 countsByYear W20348845202015 @default.
- W2034884520 countsByYear W20348845202016 @default.
- W2034884520 countsByYear W20348845202017 @default.
- W2034884520 countsByYear W20348845202018 @default.
- W2034884520 countsByYear W20348845202019 @default.
- W2034884520 countsByYear W20348845202020 @default.
- W2034884520 countsByYear W20348845202021 @default.
- W2034884520 countsByYear W20348845202022 @default.
- W2034884520 countsByYear W20348845202023 @default.
- W2034884520 crossrefType "journal-article" @default.
- W2034884520 hasAuthorship W2034884520A5024040176 @default.
- W2034884520 hasAuthorship W2034884520A5035093379 @default.
- W2034884520 hasAuthorship W2034884520A5044635824 @default.
- W2034884520 hasConcept C114246631 @default.
- W2034884520 hasConcept C16685009 @default.
- W2034884520 hasConcept C178790620 @default.
- W2034884520 hasConcept C185592680 @default.
- W2034884520 hasConcept C202751555 @default.
- W2034884520 hasConcept C2778959862 @default.
- W2034884520 hasConcept C2908647359 @default.
- W2034884520 hasConcept C29730261 @default.
- W2034884520 hasConcept C33070731 @default.
- W2034884520 hasConcept C36681516 @default.
- W2034884520 hasConcept C45080847 @default.
- W2034884520 hasConcept C54355233 @default.
- W2034884520 hasConcept C71924100 @default.
- W2034884520 hasConcept C86803240 @default.
- W2034884520 hasConcept C95444343 @default.
- W2034884520 hasConcept C99454951 @default.
- W2034884520 hasConceptScore W2034884520C114246631 @default.
- W2034884520 hasConceptScore W2034884520C16685009 @default.
- W2034884520 hasConceptScore W2034884520C178790620 @default.
- W2034884520 hasConceptScore W2034884520C185592680 @default.
- W2034884520 hasConceptScore W2034884520C202751555 @default.
- W2034884520 hasConceptScore W2034884520C2778959862 @default.
- W2034884520 hasConceptScore W2034884520C2908647359 @default.
- W2034884520 hasConceptScore W2034884520C29730261 @default.
- W2034884520 hasConceptScore W2034884520C33070731 @default.
- W2034884520 hasConceptScore W2034884520C36681516 @default.
- W2034884520 hasConceptScore W2034884520C45080847 @default.
- W2034884520 hasConceptScore W2034884520C54355233 @default.
- W2034884520 hasConceptScore W2034884520C71924100 @default.
- W2034884520 hasConceptScore W2034884520C86803240 @default.
- W2034884520 hasConceptScore W2034884520C95444343 @default.
- W2034884520 hasConceptScore W2034884520C99454951 @default.
- W2034884520 hasIssue "1-2" @default.
- W2034884520 hasLocation W20348845201 @default.
- W2034884520 hasLocation W20348845202 @default.
- W2034884520 hasOpenAccess W2034884520 @default.
- W2034884520 hasPrimaryLocation W20348845201 @default.
- W2034884520 hasRelatedWork W1888660086 @default.
- W2034884520 hasRelatedWork W1969438236 @default.
- W2034884520 hasRelatedWork W2000979784 @default.
- W2034884520 hasRelatedWork W2007201782 @default.
- W2034884520 hasRelatedWork W2028080495 @default.
- W2034884520 hasRelatedWork W2029666744 @default.
- W2034884520 hasRelatedWork W2351232732 @default.
- W2034884520 hasRelatedWork W2978891345 @default.
- W2034884520 hasRelatedWork W4295969495 @default.
- W2034884520 hasRelatedWork W2572499993 @default.
- W2034884520 hasVolume "514" @default.
- W2034884520 isParatext "false" @default.
- W2034884520 isRetracted "false" @default.
- W2034884520 magId "2034884520" @default.
- W2034884520 workType "article" @default.