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- W2034889222 abstract "Background Chlamydia trachomatis is a unique obligate intracellular bacterium that remains the leading cause of sexually transmitted bacterial diseases and preventable blindness worldwide. Chronic ocular infections are referred to as trachoma, and predominate in developing countries. Since 2001, the World Health Organization has promoted control strategies including antibiotics, improved hygiene, and environmental measures with limited success. Consequently, a vaccine is urgently needed. Integral to vaccine design is an understanding of the interactions of the pathogen and host immune response. Various animal models of trachoma show that urogenital C. trachomatis strains and other species of the family Chlamydiaceae produce severe conjunctival inflammation and scarring similar to that of the ocular C. trachomatis strains. However, we do not know the extent of organisms that may be involved in human trachoma. Furthermore, C. trachomatis heat shock protein 60 (Hsp60) has been implicated in inflammation and conjunctival scarring but the role of other Chlamydiaceae Hsp60 in disease pathogenesis has not been examined. In this study, we set out to identify whether other Chlamydiaceae species are present in trachoma, and determine their association with severity of clinical disease and with mucosal and systemic immune responses to Chlamydiaceae species-specific Hsp60 to further investigate the immunopathogenesis of this blinding disease. Methods and Findings We randomly selected nine of 49 households in a trachoma-endemic region of Nepal. Trachoma was graded, and real-time, quantitative (k)PCR was used to detect genomic DNA and cDNA (from RNA) for Chlamydiaceae ompA and 16S rRNA genes, respectively, from conjunctival swabs. IgG antibody responses to recombinant (r) Chlamydiaceae species-specific Hsp60 were determined for tears and sera. Surprisingly, all three species—C. trachomatis, Chlamydophila psittaci, and Chlamydophila pneumoniae—were detected in eight (89%) study households; one household had no members infected with C. pneumoniae. Of 80 (63%; n = 127) infected individuals, 28 (35%) had infection with C. psittaci, or C. pneumoniae, or both; single and dual infections with C. psittaci and C. pneumoniae were significantly associated with severe conjunctival inflammation (OR 4.25 [95% confidence interval (CI), 2.9–11.3], p = 0.009] as were single infections with C. trachomatis (OR 5.7 [95% CI, 3.8–10.1], p = 0.002). Of the 80 infected individuals, 75 (93.8%) were also positive for 16S rRNA by kPCR for the same organism identified by ompA. Individuals with tear IgG immunoreactivity to Chlamydiaceae rHsp60 were eight times more likely than individuals without tear immunoreactivity to be infected (95% CI 6.4–15.1; p = 0.003), 6.2 times more likely to have severe inflammation (95% CI 4.4–12.6; p = 0.001), and 5.7 times more likely to have scarring (95% CI 3.9–11.1; p = 0.019) while individuals with serum IgG immunoreactivity were 4.1 times more likely to be infected (95% CI 3.1–10.1; p = 0.014). Conclusions We provide substantial evidence for the involvement of C. psittaci and C. pneumoniae, in addition to C. trachomatis, in trachoma. The distribution of Chlamydiaceae species by household and age suggests that these infections are widespread and not just sporadic occurrences. Infection with multiple species may explain the failure to detect chlamydiae among active trachoma cases, when only C. trachomatis is assayed for, and the failure of clinically active cases to resolve their disease following what would be considered effective C. trachomatis treatment. The evidence for viable (RNA-positive) organisms of all three species in single and coinfections, the significant association of these infections with severe inflammation, and the significant association of tear and serum IgG responses to Chlamydiaceae Hsp60 with inflammation and scarring, support the role of all three species in disease pathogenesis. Thus, while our findings should be confirmed in other trachoma-endemic countries, our data suggest that a reevaluation of treatment regimens and vaccine design may be required. Understanding the full impact of Chlamydiaceae species on the epidemiology, immunopathology, and disease outcome of trachoma presents a new challenge for Chlamydiaceae research." @default.
- W2034889222 created "2016-06-24" @default.
- W2034889222 creator A5028181302 @default.
- W2034889222 creator A5049707778 @default.
- W2034889222 creator A5053390625 @default.
- W2034889222 creator A5057130526 @default.
- W2034889222 date "2008-01-03" @default.
- W2034889222 modified "2023-09-24" @default.
- W2034889222 title "Multiple Chlamydiaceae Species in Trachoma: Implications for Disease Pathogenesis and Control" @default.
- W2034889222 cites W131173259 @default.
- W2034889222 cites W1588726943 @default.
- W2034889222 cites W1597233222 @default.
- W2034889222 cites W1607829858 @default.
- W2034889222 cites W172617973 @default.
- W2034889222 cites W1823057997 @default.
- W2034889222 cites W1895970963 @default.
- W2034889222 cites W1963771197 @default.
- W2034889222 cites W1965093793 @default.
- W2034889222 cites W1965811117 @default.
- W2034889222 cites W1976708963 @default.
- W2034889222 cites W1977226442 @default.
- W2034889222 cites W1977832959 @default.
- W2034889222 cites W1985386229 @default.
- W2034889222 cites W1986367169 @default.
- W2034889222 cites W1988960137 @default.
- W2034889222 cites W1990950374 @default.
- W2034889222 cites W1992187460 @default.
- W2034889222 cites W1993644771 @default.
- W2034889222 cites W1993768789 @default.
- W2034889222 cites W1994723467 @default.
- W2034889222 cites W2009360566 @default.
- W2034889222 cites W2012257134 @default.
- W2034889222 cites W2020376820 @default.
- W2034889222 cites W2027301919 @default.
- W2034889222 cites W2028246523 @default.
- W2034889222 cites W2031240673 @default.
- W2034889222 cites W2031284222 @default.
- W2034889222 cites W2033820637 @default.
- W2034889222 cites W2045394270 @default.
- W2034889222 cites W2047686105 @default.
- W2034889222 cites W2051586631 @default.
- W2034889222 cites W2053616927 @default.
- W2034889222 cites W2058898379 @default.
- W2034889222 cites W2059106786 @default.
- W2034889222 cites W2060502171 @default.
- W2034889222 cites W2074043860 @default.
- W2034889222 cites W2076596096 @default.
- W2034889222 cites W2078542681 @default.
- W2034889222 cites W2080072086 @default.
- W2034889222 cites W2097889231 @default.
- W2034889222 cites W2098808635 @default.
- W2034889222 cites W2099564046 @default.
- W2034889222 cites W2100651011 @default.
- W2034889222 cites W2101117455 @default.
- W2034889222 cites W2102126202 @default.
- W2034889222 cites W2102222685 @default.
- W2034889222 cites W2104905222 @default.
- W2034889222 cites W2105393842 @default.
- W2034889222 cites W2105522288 @default.
- W2034889222 cites W2106678140 @default.
- W2034889222 cites W2108775663 @default.
- W2034889222 cites W2109930580 @default.
- W2034889222 cites W2114785599 @default.
- W2034889222 cites W2115780677 @default.
- W2034889222 cites W2115797552 @default.
- W2034889222 cites W2116773235 @default.
- W2034889222 cites W2117533478 @default.
- W2034889222 cites W2117929880 @default.
- W2034889222 cites W2118453530 @default.
- W2034889222 cites W2124560947 @default.
- W2034889222 cites W2126140396 @default.
- W2034889222 cites W2127354528 @default.
- W2034889222 cites W2133656633 @default.
- W2034889222 cites W2134412546 @default.
- W2034889222 cites W2135165654 @default.
- W2034889222 cites W2135908148 @default.
- W2034889222 cites W2137493675 @default.
- W2034889222 cites W2139714233 @default.
- W2034889222 cites W2140047331 @default.
- W2034889222 cites W2140703854 @default.
- W2034889222 cites W2141109556 @default.
- W2034889222 cites W2141214343 @default.
- W2034889222 cites W2142923467 @default.
- W2034889222 cites W2142954848 @default.
- W2034889222 cites W2144921938 @default.
- W2034889222 cites W2145230724 @default.
- W2034889222 cites W2145920136 @default.
- W2034889222 cites W2147577315 @default.
- W2034889222 cites W2149254783 @default.
- W2034889222 cites W2158294771 @default.
- W2034889222 cites W2158603267 @default.
- W2034889222 cites W2159716872 @default.
- W2034889222 cites W2160879512 @default.
- W2034889222 cites W2161339747 @default.
- W2034889222 cites W2164567662 @default.
- W2034889222 cites W2169440342 @default.
- W2034889222 cites W2314233087 @default.
- W2034889222 cites W2325731393 @default.