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• W2034902641 abstract "Abstract Subsequent to binding to the zona pellucida, mammalian sperm undergo a regulated sequence of events that ultimately lead to acrosomal exocytosis. Like most regulated exocytotic processes, a rise in intracellular calcium is sufficient to trigger this event although the precise mechanism of how this is achieved is still unclear. Numerous studies on mouse sperm have indicated that a voltage‐operated Ca 2+ channel plays some immediate role following sperm binding to the zona pellucida glycoprotein ZP3. However, there is also evidence that the mammalian sperm acrosome contains a high density of IP 3 receptors, suggesting that the exocytotic event involves the release of Ca 2+ from the acrosome. The release of Ca 2+ from the acrosome may directly trigger exocytosis or may activate store‐operated Ca 2+ channels in the plasma membrane. To test the hypothesis that the acrosome is an intracellular store we loaded mammalian sperm with the membrane permeant forms of three Ca 2+ ‐sensitive fluorescent indicator dyes: fura‐2, indo‐1, and Calcium Green‐5N. Fluorescence microscopy revealed that the sperm were labeled in all intracellular compartments. When fura‐2 labeled sperm were treated with 150 μM MnCl 2 to quench all fluorescence in the cytosol, or when the sperm were labeled with the low affinity dye Calcium Green‐5N, there was a large Ca 2+ signal in the acrosome. Consistent with the acrosome serving as an intracellular Ca 2+ reservoir, the addition of 20 μM thapsigargin, a potent inhibitor of the smooth endoplasmic reticular Ca 2+ ‐ATPase (SERCA), to populations of capacitated sperm resulted in nearly 100% acrosomal exocytosis within 60 min (τ 1/2 ∼ 10 min), in the absence of extracellular Ca 2+ . Additionally, treatment of sperm with 100 μM thimerosal, an IP 3 receptor agonist, also resulted in acrosomal exocytosis. Taken together, these data suggest that the mouse sperm acrosome is a Ca 2+ store that regulates its own exocytosis through an IP 3 Ca 2+ mobilization pathway. © 2004 Wiley‐Liss, Inc." @default.
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• W2034902641 date "2004-08-27" @default.
• W2034902641 modified "2023-10-14" @default.
• W2034902641 title "The acrosomal vesicle of mouse sperm is a calcium store" @default.
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• W2034902641 doi "https://doi.org/10.1002/jcp.20172" @default.
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