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• W2034931401 abstract "Poultry products are an important source of Salmonella enterica. An effective way to reduce food poisoning due to Salmonella would be to breed chickens more resistant to infection. Unfortunately host responses to Salmonella are complex with many factors involved. To learn more about responses to Salmonella in young chickens of 2 wk old, a cDNA Microarray containing 13,319 probes was performed to compare gene expression profiles between two chicken groups under control and Salmonella infected conditions. Newly hatched chickens were orally infected with S. enterica serovar Enteritidis. Since the intestine is one of the important barriers the bacteria encounter after oral inoculation, intestine gene expression was investigated at 2 wk old. There were 588 differentially expressed genes detected, of which 276 were known genes, and of the total number 266 were up-regulated and 322 were down-regulated. Differences in gene expression between the two chicken groups were found in control as well as Salmonella infected conditions indicating a difference in the intestine development between the two chicken groups which might be linked to the difference in Salmonella susceptibility. The differential expressions of 4 genes were confirmed by quantitative real-time PCR and the results indicated that the expression changes of these genes were generally consistent with the results of GeneChips. The findings in this study have lead to the identification of novel genes and possible cellular pathways, which are host dependent. Keywords: Rugao Chickens; Salmonella; GeneChips; Intestine Tissues; Gene Expression Profile; Quantitative Real-time PCR" @default.
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• W2034931401 date "2011-12-22" @default.
• W2034931401 modified "2023-10-06" @default.
• W2034931401 title "Analysis of Gene Expression Responses to a Salmonella Infection in Rugao Chicken Intestine Using GeneChips" @default.
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• W2034931401 doi "https://doi.org/10.5713/ajas.2011.11174" @default.
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