FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034969930> ?p ?o ?g. }

• W2034969930 endingPage "44" @default.
• W2034969930 startingPage "31" @default.
• W2034969930 abstract "Conventionally, a semi-quantitative microscopic nitroblue tetrazolium (NBT) assay is used to determine the production of superoxide anion (O2(-)) in various phagocytic cells. This microscopic assay is conducted by counting the cells containing blue NBT formazan deposits, which are formed by reduction of the membrane permeable, water-soluble, yellow-colored, nitroblue tetrazolium (Y-NBT) by O2(-). However, this assay is semi-quantitative and is prone to observer bias. In the present study, we modified the NBT assay by dissolving the blue formazan particles using 2M potassium hydroxide and dimethylsulfoxide and then measured its absorbance using a microplate reader at 620nm. The absorbance of dissolved NBT increased in proportion to cell number (r = 0.9907), incubation time, and stimulus concentration. To test the usefulness of this modified assay, we compared the abilities of a number of types of phagocytic cells to produce O2(-). The cells examined included murine macrophage cell lines (RAW 264.7 and J774), freshly prepared murine peritoneal macrophages and neutrophils, a human myeloid cell line (PLB-985), and freshly prepared human peripheral blood neutrophils. In addition, we demonstrate that nitric oxide produced by RAW 264.7 cells does not interfere with the modified colorimetric NBT assay. Taken together, our results indicate that the modified colorimetric NBT assay is simple, sensitive, and quantitative, and that it can be used to determine the amounts of intracellular O2(-) produced by phagocytic cells. Thus, this assay is sensitive enough to measure, quantitatively, even the small amounts of O2(-) produced in monocytes and macrophages that are not detectable by the conventional microscopic NBT assay." @default.
• W2034969930 created "2016-06-24" @default.
• W2034969930 creator A5015375775 @default.
• W2034969930 creator A5065653327 @default.
• W2034969930 creator A5080845045 @default.
• W2034969930 creator A5086100886 @default.
• W2034969930 date "2006-01-01" @default.
• W2034969930 modified "2023-10-14" @default.
• W2034969930 title "A Quantitative Nitroblue Tetrazolium Assay for Determining Intracellular Superoxide Anion Production in Phagocytic Cells" @default.
• W2034969930 cites W1551489240 @default.
• W2034969930 cites W1978948801 @default.
• W2034969930 cites W2000629029 @default.
• W2034969930 cites W2008243630 @default.
• W2034969930 cites W2009455086 @default.
• W2034969930 cites W2010630254 @default.
• W2034969930 cites W2032999996 @default.
• W2034969930 cites W2036917022 @default.
• W2034969930 cites W2060554701 @default.
• W2034969930 cites W2068263583 @default.
• W2034969930 cites W2092575774 @default.
• W2034969930 cites W2092766784 @default.
• W2034969930 cites W2098352212 @default.
• W2034969930 cites W2161486471 @default.
• W2034969930 cites W2324271478 @default.
• W2034969930 cites W2415785897 @default.
• W2034969930 cites W2462286708 @default.
• W2034969930 cites W2464100275 @default.
• W2034969930 cites W3752704 @default.
• W2034969930 cites W4249404526 @default.
• W2034969930 cites W87350884 @default.
• W2034969930 doi "https://doi.org/10.1080/15321810500403722" @default.
• W2034969930 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16450867" @default.
• W2034969930 hasPublicationYear "2006" @default.
• W2034969930 type Work @default.
• W2034969930 sameAs 2034969930 @default.
• W2034969930 citedByCount "369" @default.
• W2034969930 countsByYear W20349699302012 @default.
• W2034969930 countsByYear W20349699302013 @default.
• W2034969930 countsByYear W20349699302014 @default.
• W2034969930 countsByYear W20349699302015 @default.
• W2034969930 countsByYear W20349699302016 @default.
• W2034969930 countsByYear W20349699302017 @default.
• W2034969930 countsByYear W20349699302018 @default.
• W2034969930 countsByYear W20349699302019 @default.
• W2034969930 countsByYear W20349699302020 @default.
• W2034969930 countsByYear W20349699302021 @default.
• W2034969930 countsByYear W20349699302022 @default.
• W2034969930 countsByYear W20349699302023 @default.
• W2034969930 crossrefType "journal-article" @default.
• W2034969930 hasAuthorship W2034969930A5015375775 @default.
• W2034969930 hasAuthorship W2034969930A5065653327 @default.
• W2034969930 hasAuthorship W2034969930A5080845045 @default.
• W2034969930 hasAuthorship W2034969930A5086100886 @default.
• W2034969930 hasConcept C153911025 @default.
• W2034969930 hasConcept C181199279 @default.
• W2034969930 hasConcept C185592680 @default.
• W2034969930 hasConcept C2776892249 @default.
• W2034969930 hasConcept C2780795997 @default.
• W2034969930 hasConcept C43617362 @default.
• W2034969930 hasConcept C533411734 @default.
• W2034969930 hasConcept C54355233 @default.
• W2034969930 hasConcept C55493867 @default.
• W2034969930 hasConcept C79879829 @default.
• W2034969930 hasConcept C81885089 @default.
• W2034969930 hasConcept C86803240 @default.
• W2034969930 hasConcept C98015330 @default.
• W2034969930 hasConceptScore W2034969930C153911025 @default.
• W2034969930 hasConceptScore W2034969930C181199279 @default.
• W2034969930 hasConceptScore W2034969930C185592680 @default.
• W2034969930 hasConceptScore W2034969930C2776892249 @default.
• W2034969930 hasConceptScore W2034969930C2780795997 @default.
• W2034969930 hasConceptScore W2034969930C43617362 @default.
• W2034969930 hasConceptScore W2034969930C533411734 @default.
• W2034969930 hasConceptScore W2034969930C54355233 @default.
• W2034969930 hasConceptScore W2034969930C55493867 @default.
• W2034969930 hasConceptScore W2034969930C79879829 @default.
• W2034969930 hasConceptScore W2034969930C81885089 @default.
• W2034969930 hasConceptScore W2034969930C86803240 @default.
• W2034969930 hasConceptScore W2034969930C98015330 @default.
• W2034969930 hasIssue "1" @default.
• W2034969930 hasLocation W20349699301 @default.
• W2034969930 hasLocation W20349699302 @default.
• W2034969930 hasOpenAccess W2034969930 @default.
• W2034969930 hasPrimaryLocation W20349699301 @default.
• W2034969930 hasRelatedWork W1508785783 @default.
• W2034969930 hasRelatedWork W1985515782 @default.
• W2034969930 hasRelatedWork W2019421902 @default.
• W2034969930 hasRelatedWork W2028065729 @default.
• W2034969930 hasRelatedWork W2034116715 @default.
• W2034969930 hasRelatedWork W2095002823 @default.
• W2034969930 hasRelatedWork W2313023424 @default.
• W2034969930 hasRelatedWork W2471666874 @default.
• W2034969930 hasRelatedWork W2793210811 @default.
• W2034969930 hasRelatedWork W2913649930 @default.
• W2034969930 hasVolume "27" @default.
• W2034969930 isParatext "false" @default.
• W2034969930 isRetracted "false" @default.
• W2034969930 magId "2034969930" @default.

• next