Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034970007> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2034970007 endingPage "668a" @default.
- W2034970007 startingPage "667a" @default.
- W2034970007 abstract "Monocytes play multiple roles in the immune system, among other things, linking innate to adaptive immunity. Despite their biological importance, monocytes alone among all other phagocytes have not been investigated during the “respiratory burst” - the coordinated activation of NADPH oxidase and proton channels. Voltage gated proton current and NADPH oxidase generated electron current were measured simultaneously in human monocytes in perforated patch configuration after one to three days in culture. Upon stimulation by 60 nM phorbol myristate acetate (PMA), electron current (reflecting NADPH oxidase activity) appeared and the proton current amplitude increased. PMA slowed the kinetics of tail currents, sped the activation of outward proton current, and shifted the gH-V relationship negatively. The NADPH oxidase inhibitor diphenylene iodonium (DPI) inhibited the electron current but affected solely the tail current kinetics of the proton current. Thus, although monocytes differentiate from a different lineage than granulocytes, their responses to PMA resemble those of other phagocytes. Hydrogen peroxide (H2O2) production was recorded fluorometrically. Zinc, at concentrations that inhibit proton current, reduced H2O2 production in monocytes. This Zn2+ sensitivity resembles that seen in neutrophils and eosinophils. The dogma that extracellular glucose is necessary to support the oxidative burst of human monocytes was tested in electrophysiological and fluorescence measurements. Electron current measured in patch clamp experiments was increased 2.5 fold by adding glucose to the bath solution; proton current was unaffected. Correspondingly, H2O2 production was strongly increased and more sustained in the presence of glucose. In summary, the electrophysiological events during activation of monocytes resemble those in other phagocytes, but NADPH oxidase is more acutely dependent on the presence of glucose." @default.
- W2034970007 created "2016-06-24" @default.
- W2034970007 creator A5016708890 @default.
- W2034970007 creator A5054586736 @default.
- W2034970007 creator A5075078029 @default.
- W2034970007 date "2009-02-01" @default.
- W2034970007 modified "2023-10-14" @default.
- W2034970007 title "Electron Current and Proton Current in Activated Human Monocytes - Strong Glucose Dependence of the Electron Current" @default.
- W2034970007 doi "https://doi.org/10.1016/j.bpj.2008.12.3528" @default.
- W2034970007 hasPublicationYear "2009" @default.
- W2034970007 type Work @default.
- W2034970007 sameAs 2034970007 @default.
- W2034970007 citedByCount "3" @default.
- W2034970007 countsByYear W20349700072015 @default.
- W2034970007 crossrefType "journal-article" @default.
- W2034970007 hasAuthorship W2034970007A5016708890 @default.
- W2034970007 hasAuthorship W2034970007A5054586736 @default.
- W2034970007 hasAuthorship W2034970007A5075078029 @default.
- W2034970007 hasBestOaLocation W20349700071 @default.
- W2034970007 hasConcept C12554922 @default.
- W2034970007 hasConcept C160448771 @default.
- W2034970007 hasConcept C169760540 @default.
- W2034970007 hasConcept C181199279 @default.
- W2034970007 hasConcept C185263204 @default.
- W2034970007 hasConcept C185592680 @default.
- W2034970007 hasConcept C2777700362 @default.
- W2034970007 hasConcept C2779719074 @default.
- W2034970007 hasConcept C38485361 @default.
- W2034970007 hasConcept C48349386 @default.
- W2034970007 hasConcept C533411734 @default.
- W2034970007 hasConcept C55493867 @default.
- W2034970007 hasConcept C86803240 @default.
- W2034970007 hasConcept C95444343 @default.
- W2034970007 hasConcept C99971728 @default.
- W2034970007 hasConceptScore W2034970007C12554922 @default.
- W2034970007 hasConceptScore W2034970007C160448771 @default.
- W2034970007 hasConceptScore W2034970007C169760540 @default.
- W2034970007 hasConceptScore W2034970007C181199279 @default.
- W2034970007 hasConceptScore W2034970007C185263204 @default.
- W2034970007 hasConceptScore W2034970007C185592680 @default.
- W2034970007 hasConceptScore W2034970007C2777700362 @default.
- W2034970007 hasConceptScore W2034970007C2779719074 @default.
- W2034970007 hasConceptScore W2034970007C38485361 @default.
- W2034970007 hasConceptScore W2034970007C48349386 @default.
- W2034970007 hasConceptScore W2034970007C533411734 @default.
- W2034970007 hasConceptScore W2034970007C55493867 @default.
- W2034970007 hasConceptScore W2034970007C86803240 @default.
- W2034970007 hasConceptScore W2034970007C95444343 @default.
- W2034970007 hasConceptScore W2034970007C99971728 @default.
- W2034970007 hasIssue "3" @default.
- W2034970007 hasLocation W20349700071 @default.
- W2034970007 hasOpenAccess W2034970007 @default.
- W2034970007 hasPrimaryLocation W20349700071 @default.
- W2034970007 hasRelatedWork W1557337012 @default.
- W2034970007 hasRelatedWork W1874751834 @default.
- W2034970007 hasRelatedWork W1988681386 @default.
- W2034970007 hasRelatedWork W2012686340 @default.
- W2034970007 hasRelatedWork W2037112474 @default.
- W2034970007 hasRelatedWork W2063340476 @default.
- W2034970007 hasRelatedWork W2098954989 @default.
- W2034970007 hasRelatedWork W2100092871 @default.
- W2034970007 hasRelatedWork W2551731800 @default.
- W2034970007 hasRelatedWork W96395340 @default.
- W2034970007 hasVolume "96" @default.
- W2034970007 isParatext "false" @default.
- W2034970007 isRetracted "false" @default.
- W2034970007 magId "2034970007" @default.
- W2034970007 workType "article" @default.