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• W2034976653 abstract "Fluoride (F) absorption from the rat stomach and urinary bladder, hamster cheek pouch, and the renal tubules of several species are pH gradient-dependent. These observations led to the hypothesis that F crosses these epithelia in the form of the undissociated acid, HF. Several recent reports, however, have provided evidence that F absorption from the rat small intestine is insensitive to the lumenal pH. We report here our evidence that F uptake by rabbit intestinal brush border membrane vesicles (BBMV) occurred rapidly and with an overshoot only in the presence of an inward-directed proton gradient. In the absence of a proton gradient or in the presence of an outward-directed gradient, F uptake was slow and without an overshoot. In the presence of an inward-directed proton gradient, F uptake was partially inhibited by DIDS and DEP but not by diBAC. PCMBS inhibited F uptake by up to 83% in a dose-response manner. DiBAC appeared to reduce intravesicular pH slightly but the other reagents had no effect. When the uptake buffer contained chloride or nitrate, F uptake was partially inhibited compared to the mannitol or gluconate controls. It was concluded that F transport across the rabbit intestinal BBMV occurs via a carrier-mediated process which may involve cotransport of F with H+ or exchange of F with OH−. The inhibitory effects of DIDS, DEP and PCMBS may occur by affecting this carrier-mediated transport." @default.
• W2034976653 created "2016-06-24" @default.
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• W2034976653 date "1998-07-01" @default.
• W2034976653 modified "2023-09-25" @default.
• W2034976653 title "pH-dependent fluoride transport in intestinal brush border membrane vesicles" @default.
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• W2034976653 doi "https://doi.org/10.1016/s0005-2736(98)00064-9" @default.
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