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• W2034982415 abstract "Single-molecule fluorescence resonant energy transfer (FRET) is a widely accepted method for determining the spatial separation between molecules. In combination with pulsed interleaved excitation (PIE), additional information about the stoichiometry of molecular interactions is obtained. PIE-FRET, however, as implemented with standard confocal optics, requires the dilution of the sample to biologically low concentrations. Here, we show that PIE-FRET measurements inside nanometer-sized apertures yield meaningful biochemical data at 1000 x higher concentrations." @default.
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• W2034982415 date "2007-05-16" @default.
• W2034982415 modified "2023-10-02" @default.
• W2034982415 title "Pulsed-Interleaved Excitation FRET Measurements on Single Duplex DNA Molecules Inside C-Shaped Nanoapertures" @default.
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• W2034982415 doi "https://doi.org/10.1021/nl070822v" @default.
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