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• W2034985133 abstract "Tightly linked to the gene that encodes murine beta-glucuronidase (GUS) are three GUS-specific regulatory elements. Together, these elements define the GUS gene complex. Specific alleles of each regulatory element are associated with a specific GUS structural allele. These associations define the three common forms (haplotypes) of the GUS gene complex, designated A, B, and H. As an initial step in defining the DNA determinants of each regulatory element and to develop DNA markers for the common haplotypes, we have identified several DNA variants by blot hybridization analysis of restricted genomic DNA using GUS-specific cDNA probes. Of 30 tested restriction endonucleases, 24 reveal DNA polymorphisms that distinguish B- and H-haplotype DNA from that of the A haplotype. Of these 24, 18 uncover a restriction fragment length polymorphism in which the polymorphic fragment of A-haplotype DNA is 200-300 bp larger than the corresponding fragment of B- or H-haplotype DNA. DNA sequence analysis of this polymorphic region reveals the presence of a short, interspersed repetitive element of the B2 family within A-haplotype DNA which is absent in DNAs of B- or H-haplotype mice. None of the DNA variations revealed by these analyses can be associated at this time with variation in the regulatory or structural properties of GUS among the common haplotypes. Nevertheless, they do provide useful haplotype-specific markers within the GUS gene complex which are of critical importance for DNA transfer experiments in transgenic mice and in cultured cells." @default.
• W2034985133 created "2016-06-24" @default.
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• W2034985133 date "1987-10-01" @default.
• W2034985133 modified "2023-09-25" @default.
• W2034985133 title "DNA sequence variation within the β-glucuronidase gene complex among inbred strains of mice" @default.
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• W2034985133 doi "https://doi.org/10.1016/0888-7543(87)90006-1" @default.
• W2034985133 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2891607" @default.
• W2034985133 hasPublicationYear "1987" @default.
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