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- W2034985239 abstract "Abstract Enzymatic labeling with 18oxygen has the potential to become a widely applied method of isotope labeling for differential protein expression analysis by mass spectrometry because it is not amino acid specific and the reagents are cost‐effective and readily available. In this work, we investigate experimental parameters that affect efficient 18O incorporation with a model bovine serum albumin protein system and then use optimized chemistries for labeling the c‐terminus of peptides in a yeast proteome. Additionally, the role of sample handling, including the use of liquid chromatography was examined. An analytical methodology was developed which demonstrates the application of multi‐dimensional chromatography in conjunction with enzymatic labeling." @default.
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- W2034985239 date "2007-08-01" @default.
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- W2034985239 title "Incorporation of<sup>18</sup>Oxygen into Peptide Mixtures and Analysis with Multi‐Dimensional Chromatography and Mass‐Spectroscopy" @default.
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- W2034985239 doi "https://doi.org/10.1080/00032710701384659" @default.
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