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• W2034985449 abstract "A large number of inherited diseases are caused by premature termination codon (PTC) mutations that lead to the degradation of mRNA template. In this report, we developed a dual fluorescent reporter that relied the feature of fluorescent protein coding region to express a fusion protein from pDsRed-EGFPmtag-. Expression of the fusion protein from a single reporter provides a sensitive approach for high-throughput screening of cell-specific PTC events in mixed cell cultures. Results from the read-through analysis of COS7 cells carrying the nonsense mutation pDsRed-EGFPmtag-Y445X treated by PTC 124 showed EGFP transcript level was increased in the COS7 cells treated by PTC124 in a dose-dependent manner. This novel reporter system was applicable to the majority of different PTC patterns and could be used to quantify efficiency of read-through within a single cell or select cells carrying PTC." @default.
• W2034985449 created "2016-06-24" @default.
• W2034985449 creator A5031110143 @default.
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• W2034985449 date "2014-06-17" @default.
• W2034985449 modified "2023-10-04" @default.
• W2034985449 title "pDsRed-EGFPmtag-, an effective dual fluorescent reporter system for cell-based screens of premature termination codon" @default.
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• W2034985449 doi "https://doi.org/10.1007/s10616-014-9728-x" @default.
• W2034985449 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4628933" @default.
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• W2034985449 hasPublicationYear "2014" @default.
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