Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034985507> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2034985507 endingPage "366" @default.
- W2034985507 startingPage "359" @default.
- W2034985507 abstract "Due to the increasing evidences on dinoflagellate contribution to mucilage phenomena in the Mediterranean Sea, this study was aimed at identifying biochemical and/or genetic markers that could help in relating Gonyaulax fragilis growth with mucilage formation when microscope identification was difficult. The biochemical characterization considered the fatty acid and sterol composition of cultured strains and mucilage samples. The cultured algae displayed the typical fatty acid pattern of dinoflagellates as the 18:0 was the prevalent saturated fatty acid (SFA), 18:1n−7 and 18:1n−9 were the major monounsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs) were dominated by 18:5n−3 and 22:6n−3. Two groups of sterols were identified in algal cells: desmethylsterols, dominated by cholesterol and β-sitosterol, and 4α-methylsterols, having dinosterol as the prevailing sterol. The mucilage lipid pattern was characterized by low amounts of PUFAs and appeared deeply influenced by environment dynamics; however, the 20:5n−3/22:6n−3 ratio <1, the low C16/C18 and 16:1/16:0 ratios evidenced a higher contribution of dinoflagellates to mucilage formation than previously observed. Mucilage sterols showed higher correspondence with those found in G. fragilis than fatty acids. As a genetic marker for G. fragilis a molecular probe that could specifically bind to its 18S rDNA was designed and assayed. The probe displayed a good specificity and sensitivity as it could recognize G. fragilis DNA in the presence of high concentrations of different species, and also in samples having a high complexity and that were partially degraded, as often occurs in field mucilage samples. The results suggest that, among lipid biomarkers, sterols have a higher specificity and persistence than fatty acids and that nucleotide probes could be useful to identify this dinoflagellate in field samples, thus representing a tool to test mucilage samples of uncertain biogenetic origin." @default.
- W2034985507 created "2016-06-24" @default.
- W2034985507 creator A5018349043 @default.
- W2034985507 creator A5034884753 @default.
- W2034985507 creator A5070118530 @default.
- W2034985507 creator A5075092328 @default.
- W2034985507 creator A5075228950 @default.
- W2034985507 creator A5080305312 @default.
- W2034985507 date "2010-05-01" @default.
- W2034985507 modified "2023-09-26" @default.
- W2034985507 title "Lipid and DNA features of Gonyaulax fragilis (Dinophyceae) as potential biomarkers in mucilage genesis" @default.
- W2034985507 cites W1532082124 @default.
- W2034985507 cites W1970483911 @default.
- W2034985507 cites W1971876898 @default.
- W2034985507 cites W1975375900 @default.
- W2034985507 cites W1985651928 @default.
- W2034985507 cites W1988040485 @default.
- W2034985507 cites W1988557824 @default.
- W2034985507 cites W2011192688 @default.
- W2034985507 cites W2012792340 @default.
- W2034985507 cites W2014875317 @default.
- W2034985507 cites W2031825133 @default.
- W2034985507 cites W2032725117 @default.
- W2034985507 cites W2042101624 @default.
- W2034985507 cites W2042728276 @default.
- W2034985507 cites W2044976717 @default.
- W2034985507 cites W2061697163 @default.
- W2034985507 cites W2076691922 @default.
- W2034985507 cites W2082736251 @default.
- W2034985507 cites W2083726287 @default.
- W2034985507 cites W2085226627 @default.
- W2034985507 cites W2086003797 @default.
- W2034985507 cites W2091387367 @default.
- W2034985507 cites W2091816837 @default.
- W2034985507 cites W2095477344 @default.
- W2034985507 cites W2119077881 @default.
- W2034985507 cites W2121659710 @default.
- W2034985507 cites W2165683399 @default.
- W2034985507 cites W2168526937 @default.
- W2034985507 cites W2169556988 @default.
- W2034985507 cites W4205600055 @default.
- W2034985507 cites W4233289363 @default.
- W2034985507 doi "https://doi.org/10.1016/j.hal.2010.01.004" @default.
- W2034985507 hasPublicationYear "2010" @default.
- W2034985507 type Work @default.
- W2034985507 sameAs 2034985507 @default.
- W2034985507 citedByCount "8" @default.
- W2034985507 countsByYear W20349855072012 @default.
- W2034985507 countsByYear W20349855072013 @default.
- W2034985507 countsByYear W20349855072015 @default.
- W2034985507 countsByYear W20349855072016 @default.
- W2034985507 countsByYear W20349855072018 @default.
- W2034985507 countsByYear W20349855072023 @default.
- W2034985507 crossrefType "journal-article" @default.
- W2034985507 hasAuthorship W2034985507A5018349043 @default.
- W2034985507 hasAuthorship W2034985507A5034884753 @default.
- W2034985507 hasAuthorship W2034985507A5070118530 @default.
- W2034985507 hasAuthorship W2034985507A5075092328 @default.
- W2034985507 hasAuthorship W2034985507A5075228950 @default.
- W2034985507 hasAuthorship W2034985507A5080305312 @default.
- W2034985507 hasConcept C142796444 @default.
- W2034985507 hasConcept C18903297 @default.
- W2034985507 hasConcept C2778908408 @default.
- W2034985507 hasConcept C2780892065 @default.
- W2034985507 hasConcept C59822182 @default.
- W2034985507 hasConcept C86803240 @default.
- W2034985507 hasConcept C99696124 @default.
- W2034985507 hasConceptScore W2034985507C142796444 @default.
- W2034985507 hasConceptScore W2034985507C18903297 @default.
- W2034985507 hasConceptScore W2034985507C2778908408 @default.
- W2034985507 hasConceptScore W2034985507C2780892065 @default.
- W2034985507 hasConceptScore W2034985507C59822182 @default.
- W2034985507 hasConceptScore W2034985507C86803240 @default.
- W2034985507 hasConceptScore W2034985507C99696124 @default.
- W2034985507 hasIssue "4" @default.
- W2034985507 hasLocation W20349855071 @default.
- W2034985507 hasOpenAccess W2034985507 @default.
- W2034985507 hasPrimaryLocation W20349855071 @default.
- W2034985507 hasRelatedWork W1581937648 @default.
- W2034985507 hasRelatedWork W1972988094 @default.
- W2034985507 hasRelatedWork W2027613402 @default.
- W2034985507 hasRelatedWork W2037126417 @default.
- W2034985507 hasRelatedWork W2069532391 @default.
- W2034985507 hasRelatedWork W2078395697 @default.
- W2034985507 hasRelatedWork W2085962496 @default.
- W2034985507 hasRelatedWork W2107149713 @default.
- W2034985507 hasRelatedWork W2157339722 @default.
- W2034985507 hasRelatedWork W4252572704 @default.
- W2034985507 hasVolume "9" @default.
- W2034985507 isParatext "false" @default.
- W2034985507 isRetracted "false" @default.
- W2034985507 magId "2034985507" @default.
- W2034985507 workType "article" @default.