FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2034994702> ?p ?o ?g. }

• W2034994702 endingPage "9647" @default.
• W2034994702 startingPage "9640" @default.
• W2034994702 abstract "The function of the 102-amino acid C-terminal propeptide of surfactant protein B (SP-B) was analyzed by characterizing the phenotype associated with loss of expression of this peptide domain in transgenic mice. A construct encoding the signal peptide, N-terminal propeptide, and mature peptide of human SP-B (hSP-BΔc) was cloned under the control of the 3.7-kilobase human SP-C promoter and injected into fertilized eggs of the FVB/N mouse strain. Founder mice expressing the hSP-BΔc transgene were bred with heterozygous SP-B knockout mice (SP-B +/−). Offspring containing the transgene and one allele of mouse SP-B were identified and subsequently crossed to generate a transgenic line that expressed SP-BΔc in a null background (SP-B(−/−)/hSP-BΔc(+/+)). Expression of hSP-BΔc in SP-B(−/−) mice was restricted to type II cells and resulted in a 2-fold increase in mature SP-B relative to wild type littermates. These mice survived without any evidence of respiratory problems and had normal lung function, normal alveolar surfactant phospholipid pool sizes, and typical tubular myelin indicating that the 102-residue C-terminal propeptide of SP-B is not required for normal structure and function of extracellular surfactant. However, proteolytic processing of the SP-C proprotein was perturbed resulting in the accumulation of a processing intermediate, Mr = 11,000, similar to the phenotype detected in SP-B(−/−) mice; furthermore, lamellar bodies in type II cells of SP-B(−/−)/hSP-BΔc(+/+) mice were much larger than in the wild type animal and saturated phosphatidylcholine content in lung tissue was significantly increased although the incorporation of choline into saturated phosphatidylcholine was normal. Collectively, these results demonstrate a role for the C-terminal propeptide of SP-B in SP-C proprotein processing and the maintenance of lamellar body size. The C-terminal propeptide may be an important determinant of intracellular surfactant pool size. The function of the 102-amino acid C-terminal propeptide of surfactant protein B (SP-B) was analyzed by characterizing the phenotype associated with loss of expression of this peptide domain in transgenic mice. A construct encoding the signal peptide, N-terminal propeptide, and mature peptide of human SP-B (hSP-BΔc) was cloned under the control of the 3.7-kilobase human SP-C promoter and injected into fertilized eggs of the FVB/N mouse strain. Founder mice expressing the hSP-BΔc transgene were bred with heterozygous SP-B knockout mice (SP-B +/−). Offspring containing the transgene and one allele of mouse SP-B were identified and subsequently crossed to generate a transgenic line that expressed SP-BΔc in a null background (SP-B(−/−)/hSP-BΔc(+/+)). Expression of hSP-BΔc in SP-B(−/−) mice was restricted to type II cells and resulted in a 2-fold increase in mature SP-B relative to wild type littermates. These mice survived without any evidence of respiratory problems and had normal lung function, normal alveolar surfactant phospholipid pool sizes, and typical tubular myelin indicating that the 102-residue C-terminal propeptide of SP-B is not required for normal structure and function of extracellular surfactant. However, proteolytic processing of the SP-C proprotein was perturbed resulting in the accumulation of a processing intermediate, Mr = 11,000, similar to the phenotype detected in SP-B(−/−) mice; furthermore, lamellar bodies in type II cells of SP-B(−/−)/hSP-BΔc(+/+) mice were much larger than in the wild type animal and saturated phosphatidylcholine content in lung tissue was significantly increased although the incorporation of choline into saturated phosphatidylcholine was normal. Collectively, these results demonstrate a role for the C-terminal propeptide of SP-B in SP-C proprotein processing and the maintenance of lamellar body size. The C-terminal propeptide may be an important determinant of intracellular surfactant pool size." @default.
• W2034994702 created "2016-06-24" @default.
• W2034994702 creator A5001813162 @default.
• W2034994702 creator A5008257150 @default.
• W2034994702 creator A5025129074 @default.
• W2034994702 creator A5044828350 @default.
• W2034994702 creator A5048997124 @default.
• W2034994702 creator A5055786810 @default.
• W2034994702 creator A5076459383 @default.
• W2034994702 creator A5082463233 @default.
• W2034994702 date "1997-04-01" @default.
• W2034994702 modified "2023-10-17" @default.
• W2034994702 title "Rescue of SP-B Knockout Mice with a Truncated SP-B Proprotein" @default.
• W2034994702 cites W1513897103 @default.
• W2034994702 cites W1574356619 @default.
• W2034994702 cites W1840067061 @default.
• W2034994702 cites W1858962045 @default.
• W2034994702 cites W1983451815 @default.
• W2034994702 cites W1984626323 @default.
• W2034994702 cites W1990328256 @default.
• W2034994702 cites W1990402565 @default.
• W2034994702 cites W1996041999 @default.
• W2034994702 cites W1996421710 @default.
• W2034994702 cites W2002273560 @default.
• W2034994702 cites W2007432154 @default.
• W2034994702 cites W2035189704 @default.
• W2034994702 cites W2043563857 @default.
• W2034994702 cites W2056262259 @default.
• W2034994702 cites W2060204050 @default.
• W2034994702 cites W2067376187 @default.
• W2034994702 cites W2068808582 @default.
• W2034994702 cites W2091234795 @default.
• W2034994702 cites W2098423134 @default.
• W2034994702 cites W2133169295 @default.
• W2034994702 cites W2140288971 @default.
• W2034994702 cites W2142163437 @default.
• W2034994702 cites W2147034448 @default.
• W2034994702 cites W2179225990 @default.
• W2034994702 cites W2331089332 @default.
• W2034994702 cites W2332714852 @default.
• W2034994702 cites W2342135985 @default.
• W2034994702 cites W2409164624 @default.
• W2034994702 cites W2419144451 @default.
• W2034994702 cites W25816947 @default.
• W2034994702 doi "https://doi.org/10.1074/jbc.272.15.9640" @default.
• W2034994702 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9092492" @default.
• W2034994702 hasPublicationYear "1997" @default.
• W2034994702 type Work @default.
• W2034994702 sameAs 2034994702 @default.
• W2034994702 citedByCount "70" @default.
• W2034994702 countsByYear W20349947022012 @default.
• W2034994702 countsByYear W20349947022015 @default.
• W2034994702 countsByYear W20349947022016 @default.
• W2034994702 countsByYear W20349947022017 @default.
• W2034994702 countsByYear W20349947022018 @default.
• W2034994702 countsByYear W20349947022019 @default.
• W2034994702 countsByYear W20349947022021 @default.
• W2034994702 countsByYear W20349947022022 @default.
• W2034994702 crossrefType "journal-article" @default.
• W2034994702 hasAuthorship W2034994702A5001813162 @default.
• W2034994702 hasAuthorship W2034994702A5008257150 @default.
• W2034994702 hasAuthorship W2034994702A5025129074 @default.
• W2034994702 hasAuthorship W2034994702A5044828350 @default.
• W2034994702 hasAuthorship W2034994702A5048997124 @default.
• W2034994702 hasAuthorship W2034994702A5055786810 @default.
• W2034994702 hasAuthorship W2034994702A5076459383 @default.
• W2034994702 hasAuthorship W2034994702A5082463233 @default.
• W2034994702 hasBestOaLocation W20349947021 @default.
• W2034994702 hasConcept C102230213 @default.
• W2034994702 hasConcept C104317684 @default.
• W2034994702 hasConcept C10858879 @default.
• W2034994702 hasConcept C141035611 @default.
• W2034994702 hasConcept C153911025 @default.
• W2034994702 hasConcept C167625842 @default.
• W2034994702 hasConcept C182704531 @default.
• W2034994702 hasConcept C2779281246 @default.
• W2034994702 hasConcept C55493867 @default.
• W2034994702 hasConcept C7860815 @default.
• W2034994702 hasConcept C86803240 @default.
• W2034994702 hasConceptScore W2034994702C102230213 @default.
• W2034994702 hasConceptScore W2034994702C104317684 @default.
• W2034994702 hasConceptScore W2034994702C10858879 @default.
• W2034994702 hasConceptScore W2034994702C141035611 @default.
• W2034994702 hasConceptScore W2034994702C153911025 @default.
• W2034994702 hasConceptScore W2034994702C167625842 @default.
• W2034994702 hasConceptScore W2034994702C182704531 @default.
• W2034994702 hasConceptScore W2034994702C2779281246 @default.
• W2034994702 hasConceptScore W2034994702C55493867 @default.
• W2034994702 hasConceptScore W2034994702C7860815 @default.
• W2034994702 hasConceptScore W2034994702C86803240 @default.
• W2034994702 hasIssue "15" @default.
• W2034994702 hasLocation W20349947021 @default.
• W2034994702 hasOpenAccess W2034994702 @default.
• W2034994702 hasPrimaryLocation W20349947021 @default.
• W2034994702 hasRelatedWork W1792668954 @default.
• W2034994702 hasRelatedWork W1983947702 @default.
• W2034994702 hasRelatedWork W1985822438 @default.
• W2034994702 hasRelatedWork W1991798906 @default.

• next