FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035041872> ?p ?o ?g. }

• W2035041872 endingPage "70" @default.
• W2035041872 startingPage "57" @default.
• W2035041872 abstract "Foreign protein sequences presented on hamster polyomavirus (HaPyV) major capsid protein VP1-derived virus-like particles (VLPs) have been demonstrated to be highly immunogenic. The current study was aimed to evaluate VP1-derived chimeric VLPs as tools for hybridoma technology to generate monoclonal antibodies (mAbs) of desired specificity. Chimeric VLPs containing inserts of different size and origin were used as immunogens. Chimeric VLPs carrying a 9 amino acid (aa)-long cytotoxic T-cell epitope (STAPPVHNV) of human mucin 1 (MUC1) elicited a strong epitope-specific humoral immune response in mice and promoted the production of MUC1-specific mAbs. From a total of seven mAbs of IgG isotype generated against the chimeric VLPs, two mAbs were directed against the MUC1 epitope and five mAbs against the VP1-carrier. Two out of five anti-VP1 mAbs recognized epitopes located at the previously defined insertion site #2 (aa 223/224), which confirms its surface-exposed localization. Chimeric VLPs carrying a 120-aa long sequence of Puumala hantavirus (PUUV) nucleocapsid protein (NP) promoted the generation of five mAbs of IgG isotype specific to PUUV NP. All mAbs recognized the full-length NP of different PUUV strains. In contrast, no VP1-specific mAbs were obtained. The ability of chimeric VLPs to activate antigen-presenting cells was evaluated by studying the uptake of chimeric VLPs by murine spleen cell-derived dendritic cells (DCs). Efficient uptake of VLPs and activation of murine DCs were demonstrated, which may represent the basis of the strong immunogenicity of chimeric VLPs. In conclusion, chimeric VLPs effectively stimulated the production of IgG antibodies specific for foreign epitopes presented at surface-exposed regions. Thus, chimeric HaPyV VP1-derived VLPs represent efficient immunogens for hybridoma technology and provide a promising alternative to chemical coupling of synthetic peptides to carrier proteins." @default.
• W2035041872 created "2016-06-24" @default.
• W2035041872 creator A5044819260 @default.
• W2035041872 creator A5049618538 @default.
• W2035041872 creator A5051129046 @default.
• W2035041872 creator A5061691457 @default.
• W2035041872 creator A5069728174 @default.
• W2035041872 creator A5078412349 @default.
• W2035041872 date "2006-04-01" @default.
• W2035041872 modified "2023-09-30" @default.
• W2035041872 title "Generation of monoclonal antibodies of desired specificity using chimeric polyomavirus-derived virus-like particles" @default.
• W2035041872 cites W1518785179 @default.
• W2035041872 cites W1853664782 @default.
• W2035041872 cites W1967780063 @default.
• W2035041872 cites W1971058650 @default.
• W2035041872 cites W1972213702 @default.
• W2035041872 cites W1976801077 @default.
• W2035041872 cites W1981076153 @default.
• W2035041872 cites W1990670165 @default.
• W2035041872 cites W2000531581 @default.
• W2035041872 cites W2001085828 @default.
• W2035041872 cites W2020348374 @default.
• W2035041872 cites W2025621029 @default.
• W2035041872 cites W2027052868 @default.
• W2035041872 cites W2030522563 @default.
• W2035041872 cites W2045057463 @default.
• W2035041872 cites W2060195743 @default.
• W2035041872 cites W2068850000 @default.
• W2035041872 cites W2072938676 @default.
• W2035041872 cites W2079910321 @default.
• W2035041872 cites W2080808534 @default.
• W2035041872 cites W2083027436 @default.
• W2035041872 cites W2086724856 @default.
• W2035041872 cites W2091372450 @default.
• W2035041872 cites W2109578088 @default.
• W2035041872 cites W2109751281 @default.
• W2035041872 cites W2113929350 @default.
• W2035041872 cites W2124808556 @default.
• W2035041872 cites W2130081829 @default.
• W2035041872 cites W2136274079 @default.
• W2035041872 cites W2141888136 @default.
• W2035041872 cites W2162961064 @default.
• W2035041872 doi "https://doi.org/10.1016/j.jim.2006.01.007" @default.
• W2035041872 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16516908" @default.
• W2035041872 hasPublicationYear "2006" @default.
• W2035041872 type Work @default.
• W2035041872 sameAs 2035041872 @default.
• W2035041872 citedByCount "34" @default.
• W2035041872 countsByYear W20350418722012 @default.
• W2035041872 countsByYear W20350418722013 @default.
• W2035041872 countsByYear W20350418722014 @default.
• W2035041872 countsByYear W20350418722015 @default.
• W2035041872 countsByYear W20350418722016 @default.
• W2035041872 countsByYear W20350418722018 @default.
• W2035041872 countsByYear W20350418722021 @default.
• W2035041872 countsByYear W20350418722022 @default.
• W2035041872 countsByYear W20350418722023 @default.
• W2035041872 crossrefType "journal-article" @default.
• W2035041872 hasAuthorship W2035041872A5044819260 @default.
• W2035041872 hasAuthorship W2035041872A5049618538 @default.
• W2035041872 hasAuthorship W2035041872A5051129046 @default.
• W2035041872 hasAuthorship W2035041872A5061691457 @default.
• W2035041872 hasAuthorship W2035041872A5069728174 @default.
• W2035041872 hasAuthorship W2035041872A5078412349 @default.
• W2035041872 hasConcept C104317684 @default.
• W2035041872 hasConcept C123894998 @default.
• W2035041872 hasConcept C147483822 @default.
• W2035041872 hasConcept C153911025 @default.
• W2035041872 hasConcept C159047783 @default.
• W2035041872 hasConcept C159654299 @default.
• W2035041872 hasConcept C185592680 @default.
• W2035041872 hasConcept C195616568 @default.
• W2035041872 hasConcept C202878990 @default.
• W2035041872 hasConcept C203014093 @default.
• W2035041872 hasConcept C2522874641 @default.
• W2035041872 hasConcept C2776387124 @default.
• W2035041872 hasConcept C2777991845 @default.
• W2035041872 hasConcept C2780868878 @default.
• W2035041872 hasConcept C40767141 @default.
• W2035041872 hasConcept C542903549 @default.
• W2035041872 hasConcept C55493867 @default.
• W2035041872 hasConcept C86803240 @default.
• W2035041872 hasConceptScore W2035041872C104317684 @default.
• W2035041872 hasConceptScore W2035041872C123894998 @default.
• W2035041872 hasConceptScore W2035041872C147483822 @default.
• W2035041872 hasConceptScore W2035041872C153911025 @default.
• W2035041872 hasConceptScore W2035041872C159047783 @default.
• W2035041872 hasConceptScore W2035041872C159654299 @default.
• W2035041872 hasConceptScore W2035041872C185592680 @default.
• W2035041872 hasConceptScore W2035041872C195616568 @default.
• W2035041872 hasConceptScore W2035041872C202878990 @default.
• W2035041872 hasConceptScore W2035041872C203014093 @default.
• W2035041872 hasConceptScore W2035041872C2522874641 @default.
• W2035041872 hasConceptScore W2035041872C2776387124 @default.
• W2035041872 hasConceptScore W2035041872C2777991845 @default.
• W2035041872 hasConceptScore W2035041872C2780868878 @default.
• W2035041872 hasConceptScore W2035041872C40767141 @default.
• W2035041872 hasConceptScore W2035041872C542903549 @default.

• next