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- W2035223853 startingPage "1812" @default.
- W2035223853 abstract "Constitutive activation or overactivation of Ras signaling pathways contributes to epithelial tumorigenesis in several ways, one of which is cytoplasmic mislocalization of the cyclin-dependent kinase inhibitor p27 Kip1 (p27). We previously showed that such an effect can be mediated by activation of the Ral-GEF pathway by oncogenic N-Ras. However, the mechanism(s) leading to p27 cytoplasmic accumulation downstream of activated Ral remained unknown. Here, we report a dual regulation of p27 cellular localization by Ral downstream pathways, based on opposing effects via the Ral effectors RalBP1 and phospholipase D1 (PLD1). Because RalA and RalB are equally effective in mislocalizing both murine and human p27, we focus on RalA and murine p27, which lacks the Thr-157 phosphorylation site of human p27. In experiments based on specific RalA and p27 mutants, complemented with short hairpin RNA–mediated knockdown of Ral downstream signaling components, we show that activation of RalBP1 induces cytoplasmic accumulation of p27 and that this event requires p27 Ser-10 phosphorylation by protein kinase B/Akt. Of note, activation of PLD1 counteracts this effect in a Ser-10–independent manner. The physiological relevance of the modulation of p27 localization by Ral is demonstrated by the ability of Ral-mediated activation of the RalBP1 pathway to abrogate transforming growth factor-β–mediated growth arrest in epithelial cells." @default.
- W2035223853 created "2016-06-24" @default.
- W2035223853 creator A5004637588 @default.
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- W2035223853 creator A5043385426 @default.
- W2035223853 creator A5061336044 @default.
- W2035223853 creator A5087218848 @default.
- W2035223853 date "2013-06-01" @default.
- W2035223853 modified "2023-10-17" @default.
- W2035223853 title "Dual effects of Ral-activated pathways on p27 localization and TGF-β signaling" @default.
- W2035223853 cites W1528120845 @default.
- W2035223853 cites W1565561072 @default.
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- W2035223853 cites W1934430972 @default.
- W2035223853 cites W1968783143 @default.
- W2035223853 cites W1970615437 @default.
- W2035223853 cites W1970779989 @default.
- W2035223853 cites W1971245466 @default.
- W2035223853 cites W1971733850 @default.
- W2035223853 cites W1972429412 @default.
- W2035223853 cites W1973047647 @default.
- W2035223853 cites W1976331372 @default.
- W2035223853 cites W1978936325 @default.
- W2035223853 cites W1981517713 @default.
- W2035223853 cites W1982967858 @default.
- W2035223853 cites W1983500131 @default.
- W2035223853 cites W1984214225 @default.
- W2035223853 cites W1985906526 @default.
- W2035223853 cites W1989482277 @default.
- W2035223853 cites W1995482311 @default.
- W2035223853 cites W1996695017 @default.
- W2035223853 cites W1996938751 @default.
- W2035223853 cites W2003907692 @default.
- W2035223853 cites W2004216400 @default.
- W2035223853 cites W2009640362 @default.
- W2035223853 cites W2012983621 @default.
- W2035223853 cites W2018614002 @default.
- W2035223853 cites W2021263701 @default.
- W2035223853 cites W2021457628 @default.
- W2035223853 cites W2026004604 @default.
- W2035223853 cites W2031682344 @default.
- W2035223853 cites W2037582805 @default.
- W2035223853 cites W2038971084 @default.
- W2035223853 cites W2045571319 @default.
- W2035223853 cites W2045866614 @default.
- W2035223853 cites W2046425054 @default.
- W2035223853 cites W2049283940 @default.
- W2035223853 cites W2050202210 @default.
- W2035223853 cites W2060315334 @default.
- W2035223853 cites W2066793047 @default.
- W2035223853 cites W2067889127 @default.
- W2035223853 cites W2068876164 @default.
- W2035223853 cites W2078184757 @default.
- W2035223853 cites W2082262850 @default.
- W2035223853 cites W2085245164 @default.
- W2035223853 cites W2085391208 @default.
- W2035223853 cites W2091403769 @default.
- W2035223853 cites W2096553273 @default.
- W2035223853 cites W2096890855 @default.
- W2035223853 cites W2097829282 @default.
- W2035223853 cites W2100469448 @default.
- W2035223853 cites W2103732635 @default.
- W2035223853 cites W2104302589 @default.
- W2035223853 cites W2106877830 @default.
- W2035223853 cites W2107277218 @default.
- W2035223853 cites W2116104243 @default.
- W2035223853 cites W2121313275 @default.
- W2035223853 cites W2121619780 @default.
- W2035223853 cites W2121802410 @default.
- W2035223853 cites W2123004850 @default.
- W2035223853 cites W2124775559 @default.
- W2035223853 cites W2125143689 @default.
- W2035223853 cites W2125313133 @default.
- W2035223853 cites W2127957205 @default.
- W2035223853 cites W2135777326 @default.
- W2035223853 cites W2136940817 @default.
- W2035223853 cites W2144175203 @default.
- W2035223853 cites W2150097452 @default.
- W2035223853 cites W2152398347 @default.
- W2035223853 cites W2153604383 @default.
- W2035223853 cites W2154568166 @default.
- W2035223853 cites W2154849822 @default.
- W2035223853 cites W2161135924 @default.
- W2035223853 cites W2163931326 @default.
- W2035223853 cites W2164886325 @default.
- W2035223853 cites W2170024216 @default.
- W2035223853 cites W2170817287 @default.
- W2035223853 cites W2171852242 @default.
- W2035223853 cites W2326367330 @default.
- W2035223853 doi "https://doi.org/10.1091/mbc.e13-01-0007" @default.
- W2035223853 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3667732" @default.
- W2035223853 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23576547" @default.
- W2035223853 hasPublicationYear "2013" @default.
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