FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035228775> ?p ?o ?g. }

• W2035228775 endingPage "206" @default.
• W2035228775 startingPage "199" @default.
• W2035228775 abstract "The isolated Photobacterium phosphoreum luciferase is associated with a bound flavin designated P-flavin and tentatively identified as 6-(3-myristic acid)-FMN. Since FMN and myristic acid are products of the normal luciferase reaction, we explored the possibility that P-flavin can also be bound by luciferase from other luminous bacteria and serve as an active site probe. P-flavin has never been detected in Vibrio harveyi cells. We found that the V. harveyi luciferase binds P. phosphoreum P-flavin, at a ratio of 1 P-flavin per luciferase αβ dimer, and with concomitant absorption spectral perturbation of P-flavin, fluorescence quenching of P-flavin and luciferase, and activity inhibition of luciferase. Isolated P-flavin can be fully reduced photochemically. V. harveyi luciferase bound the oxidized P-flavin with a Kd (or Ki competitively against decanal) of 0.1–0.16 μM, which is three orders of magnitude lower than the Kd for FMN binding but similar to that of reduced FMN binding. The reduced P-flavin exhibited a Ki (competitively against the reduced FMN substrate) of 0.16 μM, also similar to the Kd for reduced FMN. Hence, the covalent attachment of myristic acid to FMN greatly and preferentially enhanced the binding of oxidized P-flavin. The dissociation of P-flavin was slow in comparison with the binding of reduced FMN and decanal substrates. Modification of the αCys106 near the active site by N-ethylmaleimide can be retarded by P-flavin. These findings indicate that P-flavin is potentially a superb active site probe for luciferase. We hypothesize that P-flavin is a by-product of luciferase generated by a side reaction which is trivial with the V. harveyi luciferase but significant in the P. phosphoreum luciferase-catalyzed reaction." @default.
• W2035228775 created "2016-06-24" @default.
• W2035228775 creator A5003712486 @default.
• W2035228775 creator A5020491026 @default.
• W2035228775 creator A5029087655 @default.
• W2035228775 date "2001-12-01" @default.
• W2035228775 modified "2023-10-07" @default.
• W2035228775 title "Characterization of the Binding of Photobacterium phosphoreum P-flavin by Vibrio harveyi Luciferase" @default.
• W2035228775 cites W1500619079 @default.
• W2035228775 cites W1514743628 @default.
• W2035228775 cites W1549578674 @default.
• W2035228775 cites W1552381026 @default.
• W2035228775 cites W1563291525 @default.
• W2035228775 cites W1608180090 @default.
• W2035228775 cites W166368677 @default.
• W2035228775 cites W1775749144 @default.
• W2035228775 cites W1815224849 @default.
• W2035228775 cites W1922706420 @default.
• W2035228775 cites W1963883369 @default.
• W2035228775 cites W1971684941 @default.
• W2035228775 cites W1989755013 @default.
• W2035228775 cites W1993562757 @default.
• W2035228775 cites W2000129783 @default.
• W2035228775 cites W2032003594 @default.
• W2035228775 cites W2037995003 @default.
• W2035228775 cites W2041797876 @default.
• W2035228775 cites W2042968800 @default.
• W2035228775 cites W2043209919 @default.
• W2035228775 cites W2046982894 @default.
• W2035228775 cites W2051110395 @default.
• W2035228775 cites W2057768509 @default.
• W2035228775 cites W2063518239 @default.
• W2035228775 cites W2072207841 @default.
• W2035228775 cites W2087833558 @default.
• W2035228775 cites W2090502508 @default.
• W2035228775 cites W2114035675 @default.
• W2035228775 cites W2157274822 @default.
• W2035228775 cites W2159034704 @default.
• W2035228775 cites W2175011977 @default.
• W2035228775 cites W2220844760 @default.
• W2035228775 cites W4252988873 @default.
• W2035228775 doi "https://doi.org/10.1006/abbi.2001.2612" @default.
• W2035228775 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11747297" @default.
• W2035228775 hasPublicationYear "2001" @default.
• W2035228775 type Work @default.
• W2035228775 sameAs 2035228775 @default.
• W2035228775 citedByCount "8" @default.
• W2035228775 countsByYear W20352287752015 @default.
• W2035228775 countsByYear W20352287752017 @default.
• W2035228775 countsByYear W20352287752019 @default.
• W2035228775 countsByYear W20352287752020 @default.
• W2035228775 countsByYear W20352287752021 @default.
• W2035228775 crossrefType "journal-article" @default.
• W2035228775 hasAuthorship W2035228775A5003712486 @default.
• W2035228775 hasAuthorship W2035228775A5020491026 @default.
• W2035228775 hasAuthorship W2035228775A5029087655 @default.
• W2035228775 hasConcept C104317684 @default.
• W2035228775 hasConcept C111335760 @default.
• W2035228775 hasConcept C178790620 @default.
• W2035228775 hasConcept C181199279 @default.
• W2035228775 hasConcept C185592680 @default.
• W2035228775 hasConcept C2776154503 @default.
• W2035228775 hasConcept C2778483698 @default.
• W2035228775 hasConcept C2779925203 @default.
• W2035228775 hasConcept C2779936771 @default.
• W2035228775 hasConcept C2780437130 @default.
• W2035228775 hasConcept C2781275326 @default.
• W2035228775 hasConcept C29730261 @default.
• W2035228775 hasConcept C33093398 @default.
• W2035228775 hasConcept C523546767 @default.
• W2035228775 hasConcept C54009773 @default.
• W2035228775 hasConcept C543025807 @default.
• W2035228775 hasConcept C54355233 @default.
• W2035228775 hasConcept C55493867 @default.
• W2035228775 hasConcept C71240020 @default.
• W2035228775 hasConcept C86803240 @default.
• W2035228775 hasConceptScore W2035228775C104317684 @default.
• W2035228775 hasConceptScore W2035228775C111335760 @default.
• W2035228775 hasConceptScore W2035228775C178790620 @default.
• W2035228775 hasConceptScore W2035228775C181199279 @default.
• W2035228775 hasConceptScore W2035228775C185592680 @default.
• W2035228775 hasConceptScore W2035228775C2776154503 @default.
• W2035228775 hasConceptScore W2035228775C2778483698 @default.
• W2035228775 hasConceptScore W2035228775C2779925203 @default.
• W2035228775 hasConceptScore W2035228775C2779936771 @default.
• W2035228775 hasConceptScore W2035228775C2780437130 @default.
• W2035228775 hasConceptScore W2035228775C2781275326 @default.
• W2035228775 hasConceptScore W2035228775C29730261 @default.
• W2035228775 hasConceptScore W2035228775C33093398 @default.
• W2035228775 hasConceptScore W2035228775C523546767 @default.
• W2035228775 hasConceptScore W2035228775C54009773 @default.
• W2035228775 hasConceptScore W2035228775C543025807 @default.
• W2035228775 hasConceptScore W2035228775C54355233 @default.
• W2035228775 hasConceptScore W2035228775C55493867 @default.
• W2035228775 hasConceptScore W2035228775C71240020 @default.
• W2035228775 hasConceptScore W2035228775C86803240 @default.
• W2035228775 hasIssue "2" @default.
• W2035228775 hasLocation W20352287751 @default.

• next