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• W2035230921 abstract "You have accessJournal of UrologyPediatrics: Basic Research1 Apr 2012736 URINE DERIVED STEM CELLS WITH HIGH TELOMERASE ACTIVITY FOR CELL BASED THERAPY IN UROLOGY Yingai Shi, Guihua Liu, Bharadwaj Shantaram, Anthony Atala, and Yuanyuan Zhang Yingai ShiYingai Shi Winston Salem, NC More articles by this author , Guihua LiuGuihua Liu Winston Salem, NC More articles by this author , Bharadwaj ShantaramBharadwaj Shantaram Winston Salem, NC More articles by this author , Anthony AtalaAnthony Atala Winston Salem, NC More articles by this author , and Yuanyuan ZhangYuanyuan Zhang Winston Salem, NC More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2012.02.821AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Maintenance of telomere length by telomerase is an indicator of self-renewing cells, and telomerase is commonly expressed by embryonic stem cells and some types of mesenchymal stem cells (MSC). The purpose of this study was to evaluate whether telomerase activity can be detected in urine derived stem cells (USC) derived from donors of various ages, and to determine whether USC that express telomerase possess greater proliferative capacity than cells that do not express telomerase. If so, telomerase-expressing USCs could be optimized for use as a cell source for cell based therapy in urology. METHODS One hundred and fourteen USC clones were isolated from the urine of 10 donors (ages ranging from 20-50 years) and cultured. The telomerase activities of these USC and human bone marrow stromal cells (BMSC) were measured at p2, 7, and 11 using a TRAP-ELISA assay. The proliferative capacity of the USC was also assessed by producing a growth curve over 7 days and performing population doubling and doubling time analyses. Additionally, karyotypes of four different USC clones were determined at p5. Stem cell surface markers expressed by the USC clones were evaluated by flow cytometry. RESULTS Eighty out of 114 independent USC clones (70%) isolated from the urine of 10 donors of different ages displayed detectable levels of telomerase activity (TA+) at p2. Telomerase activity decreased gradually with culture passages. The cell growth curves showed that the TA+-cell clones grew more rapidly than the TA--cell clones. TA+-cell clones demonstrated more population doublings (PD) and shorter doubling times (DT) than TA--cell clones regardless of the age of the donor. The average PD was 60.30±5.1in TA+-USC compared to 37.6 ±2.9 that in TA--USC. The average DT was 26.9±2.0 h in TA+-USC compared to 35.9 ± 1.4 h in TA--USC. The TA+-cell clones reached p11 and maintained their original ′rice-grain like′morphology throughout all passages. However, the TA--cell clones began to display a larger, flattened morphology with cellular projections at p8 to 9. The karyotype of all USCs was normal and did not appear to depend on TA level. Both TA+-USC and TA--USC expressed stem cell markers, including CD44, CD90, CD73, CD105, and CD146. However, these cells did not express CD31, CD34, CD45, CD25, CD 117, or CD140b. CONCLUSIONS USC that expressed telomerase activity possessed higher proliferative capacities and could be maintained for a higher number of passages. This information further enhances our characterization of these cells and supports the potential clinical utility of these cells in regenerative medicine in urology. © 2012 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 187Issue 4SApril 2012Page: e302 Advertisement Copyright & Permissions© 2012 by American Urological Association Education and Research, Inc.MetricsAuthor Information Yingai Shi Winston Salem, NC More articles by this author Guihua Liu Winston Salem, NC More articles by this author Bharadwaj Shantaram Winston Salem, NC More articles by this author Anthony Atala Winston Salem, NC More articles by this author Yuanyuan Zhang Winston Salem, NC More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ..." @default.
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• W2035230921 title "736 URINE DERIVED STEM CELLS WITH HIGH TELOMERASE ACTIVITY FOR CELL BASED THERAPY IN UROLOGY" @default.
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