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- W2035240410 abstract "A binary affinity chromatography medium was prepared and found to be useful for the purification and quantitative isolation of glycogen phosphorylase from rabbit skeletal muscle and liver. Glycogen is used as the binary ligand as it has affinity toward both the column matrix and the enzyme. Agarose beads derivatized with concanavalin A bound glycogen to the level of 35 mg/ml. The glycogen-impregnated beads were able to bind 9 mg/ml of phosphorylase a or b. The phosphorylase is tightly bound so that the column can be washed free of contaminants before quantitative elution of the phosphorylase by 2 M glucose, which releases the glycogen-phosphorylase complex. It appears that binary affinity chromatography may have general utility for the isolation and purification of enzymes and other specific binding agents." @default.
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- W2035240410 date "1990-11-01" @default.
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- W2035240410 title "Binary affinity chromatography for the purification of glycogen phosphorylase" @default.
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- W2035240410 doi "https://doi.org/10.1016/1046-5928(90)90007-l" @default.
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