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• W2035241014 startingPage "932" @default.
• W2035241014 abstract "Amyloid fibrils formed by the 29-residue peptide hormone glucagon at different concentrations have strikingly different morphologies when observed by transmission electron microscopy. Fibrils formed at low concentration (0.25 mg/mL) consist of two or more protofilaments with a regular twist, while fibrils at high concentration (8 mg/mL) consist of two straight protofilaments. Here, we explore the structural differences underlying glucagon polymorphism using proteolytic degradation, linear and circular dichroism, Fourier transform infrared spectroscopy (FTIR), and X-ray fiber diffraction. Morphological differences are perpetuated at all structural levels, indicating that the two fibril classes differ in terms of protofilament backbone regions, secondary structure, chromophore alignment along the fibril axis, and fibril superstructure. Straight fibrils show a conventional β-sheet-rich far-UV circular dichroism spectrum whereas that of twisted fibrils is dominated by contributions from β-turns. Fourier transform infrared spectroscopy confirms this and also indicates a more dense backbone with weaker hydrogen bonding for the twisted morphology. According to linear dichroism, the secondary structural elements and the aromatic side chains in the straight fibrils are more highly ordered with respect to the alignment axis than the twisted fibrils. A series of highly periodical reflections in the diffractogram of the straight fibrils can be fitted to the diffraction pattern expected from a cylinder. Thus, the highly integrated structural organization in the straight fibril leads to a compact and highly uniform fibril with a well-defined edge. Prolonged proteolytic digestion confirmed that the straight fibrils are very compact and stable, while parts of the twisted fibril backbone are much more readily degraded. Differences in the digest patterns of the two morphologies correlate with predictions from two algorithms, suggesting that the polymorphism is inherent in the glucagon sequence. Glucagon provides a striking illustration of how the same short sequence can be folded into two remarkably different fibrillar structures." @default.
• W2035241014 created "2016-06-24" @default.
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• W2035241014 date "2010-04-01" @default.
• W2035241014 modified "2023-10-11" @default.
• W2035241014 title "Glucagon Fibril Polymorphism Reflects Differences in Protofilament Backbone Structure" @default.
• W2035241014 cites W1856532105 @default.
• W2035241014 cites W1967078910 @default.
• W2035241014 cites W1967701034 @default.
• W2035241014 cites W1970042842 @default.
• W2035241014 cites W1970898345 @default.
• W2035241014 cites W1971122208 @default.
• W2035241014 cites W1973134139 @default.
• W2035241014 cites W1976103641 @default.
• W2035241014 cites W1976352490 @default.
• W2035241014 cites W1976548137 @default.
• W2035241014 cites W1977646659 @default.
• W2035241014 cites W1978959047 @default.
• W2035241014 cites W1979025995 @default.
• W2035241014 cites W1982005407 @default.
• W2035241014 cites W1985121447 @default.
• W2035241014 cites W1986735189 @default.
• W2035241014 cites W1990712184 @default.
• W2035241014 cites W1991230220 @default.
• W2035241014 cites W1992045220 @default.
• W2035241014 cites W1998355107 @default.
• W2035241014 cites W1998750365 @default.
• W2035241014 cites W2004098528 @default.
• W2035241014 cites W2004470794 @default.
• W2035241014 cites W2016411740 @default.
• W2035241014 cites W2019743990 @default.
• W2035241014 cites W2024410296 @default.
• W2035241014 cites W2027038277 @default.
• W2035241014 cites W2027839322 @default.
• W2035241014 cites W2029018340 @default.
• W2035241014 cites W2034951358 @default.
• W2035241014 cites W2038197478 @default.
• W2035241014 cites W2040877109 @default.
• W2035241014 cites W2047622014 @default.
• W2035241014 cites W2049695588 @default.
• W2035241014 cites W2049937327 @default.
• W2035241014 cites W2060287241 @default.
• W2035241014 cites W2061627162 @default.
• W2035241014 cites W2062593465 @default.
• W2035241014 cites W2063627711 @default.
• W2035241014 cites W2063826033 @default.
• W2035241014 cites W2064396222 @default.
• W2035241014 cites W2067214887 @default.
• W2035241014 cites W2067924797 @default.
• W2035241014 cites W2071095038 @default.
• W2035241014 cites W2074713226 @default.
• W2035241014 cites W2075482445 @default.
• W2035241014 cites W2076334645 @default.
• W2035241014 cites W2076399332 @default.
• W2035241014 cites W2076468390 @default.
• W2035241014 cites W2078115857 @default.
• W2035241014 cites W2083739589 @default.
• W2035241014 cites W2085899859 @default.
• W2035241014 cites W2098435874 @default.
• W2035241014 cites W2100629874 @default.
• W2035241014 cites W2103622805 @default.
• W2035241014 cites W2117537971 @default.
• W2035241014 cites W2121897932 @default.
• W2035241014 cites W2123613399 @default.
• W2035241014 cites W2135007733 @default.
• W2035241014 cites W2137573587 @default.
• W2035241014 cites W2143073331 @default.
• W2035241014 cites W2143495798 @default.
• W2035241014 cites W2149975011 @default.
• W2035241014 cites W2151881328 @default.
• W2035241014 cites W2154907250 @default.
• W2035241014 cites W2155916246 @default.
• W2035241014 cites W2158119813 @default.
• W2035241014 cites W2163981524 @default.
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• W2035241014 doi "https://doi.org/10.1016/j.jmb.2010.02.012" @default.
• W2035241014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20156459" @default.
• W2035241014 hasPublicationYear "2010" @default.
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