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- W2035286881 abstract "Sulfate ion produced little or no interference in absorption by sodium, potassium, and magnesium, but produced a large depression in calcium absorbance in the atomic absorption spectrophotometric measurement of these cations in an acetylene-air flame. Nearly maximal depression of calcium absorbance by 2 mM sulfate was followed by a plateau region of only slight depression from 2 mM to 1 M sulfate concentration. Presence of 25 mM lanthanum in the samples resulted in no depression of calcium absorbance up to 2 mM sulfate, a sharp decrease to about 30 mM sulfate and a plateau from 30 mM up to 1 M sulfate. From these observations, it was determined that the addition of H2SO4 to provide approximately 40 mM added sulfate in standards and samples permitted accurate measurement of calcium even though the original sample contained relatively high and variable sulfate. The results provided the basis for a practical method for the measurement of sodium, potassium, magnesium, and calcium in Kjeldahl digests of biological materials. The optimum condition for analysis was determined to be addition of reagents to give 25 mM lanthanum, 4 mM cesium, 300 mM hydrogen ion, and 40 mM sulfate in standards and samples for analysis in an acetylene-air flame by atomic absorption spectrophotometry. Application of this method to a study of the recovery of cations added to dialyzed rat liver homogenate demonstrated that sodium, potassium, magnesium, and calcium could be accurately measured in Kjeldahl digests, which could also be used for the measurement of total nitrogen." @default.
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- W2035286881 date "1972-05-01" @default.
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- W2035286881 title "An atomic absorption method for cation measurements in Kjeldahl digests of biological materials" @default.
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- W2035286881 doi "https://doi.org/10.1016/0003-2697(72)90278-3" @default.
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