Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035393952> ?p ?o ?g. }
- W2035393952 endingPage "7" @default.
- W2035393952 startingPage "1" @default.
- W2035393952 abstract "Context: One approach to protect human skin against the dangerous effects of solar ultraviolet (UV) irradiation is the use of natural products, such as photoprotectors. Phyllanthus orbicularis Kunth (Euphorbiaceae) is a Cuban endemic plant used in popular medicine. Its antigenotoxicity effect against some harmful agents has been investigated. However, the effect in ultraviolet B (UVB)-irradiated human cells has not been previously assessed.Objective: The protective effect of a P. orbicularis extract against UVB light-induced damage in human cells was evaluated.Materials and methods: DNA repair proficient (MRC5-SV) and deficient (XP4PA, complementation group XPC) cell-lines were used. Damaging effects of UVB light were evaluated by clonogenic assay and apoptosis induction by flow cytometry techniques. The extent of DNA repair itself was determined by the removal of cyclobutane pyrimidine dimers (CPDs). The CPDs were detected and quantified by slot-blot assay.Results: Treatment of UVB-irradiated MRC5-SV cells with P. orbicularis extract increased the percentage of colony-forming cells from 36.03 ± 3.59 and 4.42 ± 1.45 to 53.14 ± 8.8 and 14.52 ± 1.97, for 400 and 600 J/m2, respectively. A decrease in apoptotic cell population was observed in cells maintained within the extract. The P. orbicularis extract enhanced the removal of CPD from genomic DNA. The CPDs remaining were found to be about 27.7 and 1.1%, while with plant extract, treatment these values decreased to 16.1 and 0.2%, for 3 and 24 h, respectively.Discussion and conclusion: P. orbicularis aqueous extract protects human cells against UVB damage. This protective effect is through the modulation of DNA repair effectiveness." @default.
- W2035393952 created "2016-06-24" @default.
- W2035393952 creator A5005476587 @default.
- W2035393952 creator A5025032657 @default.
- W2035393952 creator A5040941890 @default.
- W2035393952 creator A5040974590 @default.
- W2035393952 creator A5059763911 @default.
- W2035393952 creator A5077695105 @default.
- W2035393952 creator A5079483159 @default.
- W2035393952 date "2012-12-18" @default.
- W2035393952 modified "2023-10-17" @default.
- W2035393952 title "Protective effect of a<i>Phyllanthus orbicularis</i>aqueous extract against UVB light in human cells" @default.
- W2035393952 cites W1523021484 @default.
- W2035393952 cites W1977044585 @default.
- W2035393952 cites W1984055188 @default.
- W2035393952 cites W1988093796 @default.
- W2035393952 cites W2003911732 @default.
- W2035393952 cites W2005570632 @default.
- W2035393952 cites W2006940399 @default.
- W2035393952 cites W2007034730 @default.
- W2035393952 cites W2007372884 @default.
- W2035393952 cites W2011500440 @default.
- W2035393952 cites W2016763652 @default.
- W2035393952 cites W2021816070 @default.
- W2035393952 cites W2025910569 @default.
- W2035393952 cites W2033969276 @default.
- W2035393952 cites W2042442020 @default.
- W2035393952 cites W2044428414 @default.
- W2035393952 cites W2053483375 @default.
- W2035393952 cites W2073405097 @default.
- W2035393952 cites W2074511980 @default.
- W2035393952 cites W2080605611 @default.
- W2035393952 cites W2082385133 @default.
- W2035393952 cites W2088931089 @default.
- W2035393952 cites W2090978578 @default.
- W2035393952 cites W2100130506 @default.
- W2035393952 cites W2104133183 @default.
- W2035393952 cites W2107418455 @default.
- W2035393952 cites W2109641347 @default.
- W2035393952 cites W2127390105 @default.
- W2035393952 cites W2132834292 @default.
- W2035393952 cites W2146218187 @default.
- W2035393952 cites W2149889657 @default.
- W2035393952 cites W2167036096 @default.
- W2035393952 cites W4205117617 @default.
- W2035393952 doi "https://doi.org/10.3109/13880209.2012.695800" @default.
- W2035393952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23249398" @default.
- W2035393952 hasPublicationYear "2012" @default.
- W2035393952 type Work @default.
- W2035393952 sameAs 2035393952 @default.
- W2035393952 citedByCount "9" @default.
- W2035393952 countsByYear W20353939522013 @default.
- W2035393952 countsByYear W20353939522016 @default.
- W2035393952 countsByYear W20353939522017 @default.
- W2035393952 countsByYear W20353939522018 @default.
- W2035393952 countsByYear W20353939522019 @default.
- W2035393952 countsByYear W20353939522022 @default.
- W2035393952 crossrefType "journal-article" @default.
- W2035393952 hasAuthorship W2035393952A5005476587 @default.
- W2035393952 hasAuthorship W2035393952A5025032657 @default.
- W2035393952 hasAuthorship W2035393952A5040941890 @default.
- W2035393952 hasAuthorship W2035393952A5040974590 @default.
- W2035393952 hasAuthorship W2035393952A5059763911 @default.
- W2035393952 hasAuthorship W2035393952A5077695105 @default.
- W2035393952 hasAuthorship W2035393952A5079483159 @default.
- W2035393952 hasConcept C117262875 @default.
- W2035393952 hasConcept C143425029 @default.
- W2035393952 hasConcept C151730666 @default.
- W2035393952 hasConcept C153911025 @default.
- W2035393952 hasConcept C167975912 @default.
- W2035393952 hasConcept C185592680 @default.
- W2035393952 hasConcept C190283241 @default.
- W2035393952 hasConcept C2779343474 @default.
- W2035393952 hasConcept C2908647359 @default.
- W2035393952 hasConcept C2987116853 @default.
- W2035393952 hasConcept C552990157 @default.
- W2035393952 hasConcept C553184892 @default.
- W2035393952 hasConcept C55493867 @default.
- W2035393952 hasConcept C556039675 @default.
- W2035393952 hasConcept C71924100 @default.
- W2035393952 hasConcept C75473681 @default.
- W2035393952 hasConcept C86803240 @default.
- W2035393952 hasConcept C99454951 @default.
- W2035393952 hasConceptScore W2035393952C117262875 @default.
- W2035393952 hasConceptScore W2035393952C143425029 @default.
- W2035393952 hasConceptScore W2035393952C151730666 @default.
- W2035393952 hasConceptScore W2035393952C153911025 @default.
- W2035393952 hasConceptScore W2035393952C167975912 @default.
- W2035393952 hasConceptScore W2035393952C185592680 @default.
- W2035393952 hasConceptScore W2035393952C190283241 @default.
- W2035393952 hasConceptScore W2035393952C2779343474 @default.
- W2035393952 hasConceptScore W2035393952C2908647359 @default.
- W2035393952 hasConceptScore W2035393952C2987116853 @default.
- W2035393952 hasConceptScore W2035393952C552990157 @default.
- W2035393952 hasConceptScore W2035393952C553184892 @default.
- W2035393952 hasConceptScore W2035393952C55493867 @default.
- W2035393952 hasConceptScore W2035393952C556039675 @default.
- W2035393952 hasConceptScore W2035393952C71924100 @default.