FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035408510> ?p ?o ?g. }

• W2035408510 endingPage "14102" @default.
• W2035408510 startingPage "14093" @default.
• W2035408510 abstract "Catalase-peroxidases are bifunctional heme enzymes with a high structural homology to peroxidases from prokaryotic origin and a catalatic activity comparable to monofunctional catalases. These unique features of catalase-peroxidases make them good systems to study and understand the role of alternative electron pathways both in catalases and peroxidases. In particular, it is of interest to study the poorly understood role of tyrosyl and tryptophanyl radicals as alternative cofactors in the catalytic cycle of catalases and peroxidases. In this work, we have used a powerful combination of multifrequency EPR spectroscopy, isotopic labeling of tryptophan and tyrosine residues, and site-directed mutagenesis to unequivocally identify the reactive intermediates formed by the wild-type Synechocystis PCC6803 catalase-peroxidase. Selected variants of the heme distal and proximal sides of the Synechocystis enzyme were investigated. Variants on the aromatic residues of the short stretch located relatively close to the heme and spanning the distal and proximal sides were also investigated. In the wild-type enzyme, the EPR signal of the catalases and peroxidases (typical) Compound I intermediate [Fe(IV)=O por.+] was observed. Two protein-based radical intermediates were also detected and identified as a Tyr. and a Trp. . The site of Trp. is proposed to be Trp 106, a residue belonging to the conserved short stretch in catalase-peroxidases and located at a 7-8 A distance to the heme propionate groups. An extensive hydrogen-bonding network on the heme distal side, involving Trp122, His123, Arg119, seven structural waters, the heme 6-propionate group, and Trp106, is proposed to have a key role on the formation of the tryptophanyl radical. We used high-field EPR spectroscopy (95-285 GHz) to resolve the g-anisotropy of the protein-based radicals in Synechocystis catalase-peroxidase. The broad gx component of the HF EPR spectrum of the Tyr. in Synechocystis catalase-peroxidase was consistent with a distributed electropositive protein environment to the tyrosyl radical." @default.
• W2035408510 created "2016-06-24" @default.
• W2035408510 creator A5016279871 @default.
• W2035408510 creator A5018595478 @default.
• W2035408510 creator A5044806177 @default.
• W2035408510 creator A5073002693 @default.
• W2035408510 creator A5079435712 @default.
• W2035408510 date "2003-10-24" @default.
• W2035408510 modified "2023-10-18" @default.
• W2035408510 title "Protein-Based Radicals in the Catalase-Peroxidase of <i>Synechocystis</i> PCC6803: A Multifrequency EPR Investigation of Wild-Type and Variants on the Environment of the Heme Active Site" @default.
• W2035408510 cites W1543953582 @default.
• W2035408510 cites W1964796065 @default.
• W2035408510 cites W1967072344 @default.
• W2035408510 cites W1980073565 @default.
• W2035408510 cites W1982083268 @default.
• W2035408510 cites W1983665042 @default.
• W2035408510 cites W1983974110 @default.
• W2035408510 cites W1986432247 @default.
• W2035408510 cites W1986555572 @default.
• W2035408510 cites W1993090378 @default.
• W2035408510 cites W2000504214 @default.
• W2035408510 cites W2010649008 @default.
• W2035408510 cites W2020539129 @default.
• W2035408510 cites W2021872718 @default.
• W2035408510 cites W2024327307 @default.
• W2035408510 cites W2046285733 @default.
• W2035408510 cites W2048919757 @default.
• W2035408510 cites W2051629625 @default.
• W2035408510 cites W2063473248 @default.
• W2035408510 cites W2078023361 @default.
• W2035408510 cites W2078996434 @default.
• W2035408510 cites W2085266874 @default.
• W2035408510 cites W2085886433 @default.
• W2035408510 cites W2086219034 @default.
• W2035408510 cites W2087790671 @default.
• W2035408510 cites W2091091295 @default.
• W2035408510 cites W2107277439 @default.
• W2035408510 cites W2112054055 @default.
• W2035408510 cites W304944753 @default.
• W2035408510 doi "https://doi.org/10.1021/ja035582+" @default.
• W2035408510 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14611246" @default.
• W2035408510 hasPublicationYear "2003" @default.
• W2035408510 type Work @default.
• W2035408510 sameAs 2035408510 @default.
• W2035408510 citedByCount "107" @default.
• W2035408510 countsByYear W20354085102012 @default.
• W2035408510 countsByYear W20354085102013 @default.
• W2035408510 countsByYear W20354085102014 @default.
• W2035408510 countsByYear W20354085102015 @default.
• W2035408510 countsByYear W20354085102016 @default.
• W2035408510 countsByYear W20354085102017 @default.
• W2035408510 countsByYear W20354085102018 @default.
• W2035408510 countsByYear W20354085102020 @default.
• W2035408510 countsByYear W20354085102021 @default.
• W2035408510 countsByYear W20354085102022 @default.
• W2035408510 crossrefType "journal-article" @default.
• W2035408510 hasAuthorship W2035408510A5016279871 @default.
• W2035408510 hasAuthorship W2035408510A5018595478 @default.
• W2035408510 hasAuthorship W2035408510A5044806177 @default.
• W2035408510 hasAuthorship W2035408510A5073002693 @default.
• W2035408510 hasAuthorship W2035408510A5079435712 @default.
• W2035408510 hasConcept C104317684 @default.
• W2035408510 hasConcept C133166286 @default.
• W2035408510 hasConcept C143065580 @default.
• W2035408510 hasConcept C162008176 @default.
• W2035408510 hasConcept C181199279 @default.
• W2035408510 hasConcept C185592680 @default.
• W2035408510 hasConcept C197957613 @default.
• W2035408510 hasConcept C20705724 @default.
• W2035408510 hasConcept C2776217839 @default.
• W2035408510 hasConcept C2776706248 @default.
• W2035408510 hasConcept C2778979269 @default.
• W2035408510 hasConcept C2780238584 @default.
• W2035408510 hasConcept C41183919 @default.
• W2035408510 hasConcept C515207424 @default.
• W2035408510 hasConcept C55493867 @default.
• W2035408510 hasConcept C58431802 @default.
• W2035408510 hasConcept C71240020 @default.
• W2035408510 hasConceptScore W2035408510C104317684 @default.
• W2035408510 hasConceptScore W2035408510C133166286 @default.
• W2035408510 hasConceptScore W2035408510C143065580 @default.
• W2035408510 hasConceptScore W2035408510C162008176 @default.
• W2035408510 hasConceptScore W2035408510C181199279 @default.
• W2035408510 hasConceptScore W2035408510C185592680 @default.
• W2035408510 hasConceptScore W2035408510C197957613 @default.
• W2035408510 hasConceptScore W2035408510C20705724 @default.
• W2035408510 hasConceptScore W2035408510C2776217839 @default.
• W2035408510 hasConceptScore W2035408510C2776706248 @default.
• W2035408510 hasConceptScore W2035408510C2778979269 @default.
• W2035408510 hasConceptScore W2035408510C2780238584 @default.
• W2035408510 hasConceptScore W2035408510C41183919 @default.
• W2035408510 hasConceptScore W2035408510C515207424 @default.
• W2035408510 hasConceptScore W2035408510C55493867 @default.
• W2035408510 hasConceptScore W2035408510C58431802 @default.
• W2035408510 hasConceptScore W2035408510C71240020 @default.
• W2035408510 hasIssue "46" @default.
• W2035408510 hasLocation W20354085101 @default.
• W2035408510 hasLocation W20354085102 @default.

• next