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• W2035435138 abstract "In our previous study, we reported an increase in the thermal stability of bacterial laccase from Bacillus sp. HR03 using site‐directed mutagenesis. Three‐dimensional model of this enzyme showed a negative patch in the connecting loop between domains 1 and 2. In the present study, the stability of laccase in organic solvents was improved by introducing nonpolar (E188 → A, I, L, and V) and positively charged (E188 → K and R) residues in this region by site‐directed mutagenesis. Irreversible thermoinactivation, C 50 value (organic solvent concentration at which 50% of enzyme activity remains), change in transition‐state stabilization energy, and kinetic parameters of the wild type and its variants were calculated in the presence and absence of various organic solvents (ethanol, methanol, and 1‐propanol). All variants showed higher C 50 values when compared to the wild type. Nonpolar amino acid substitutions were found to be the most efficient mutants for their remarkable increase in C 50 value and a decrease in thermoinactivation rate in the presence of mentioned solvents. Data showed that replacing a negative residue with hydrophobic residues on the surface of a protein could enhance thermoresistance as well as solvent stability. The stability of the resulting enzymes was dependent on the length of the alkyl chain. Results demonstrated that solvent tolerance was positively correlated with thermal stability." @default.
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• W2035435138 date "2014-05-11" @default.
• W2035435138 modified "2023-10-16" @default.
• W2035435138 title "Protein engineering of laccase to enhance its activity and stability in the presence of organic solvents" @default.
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• W2035435138 doi "https://doi.org/10.1002/elsc.201300042" @default.
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