Matches in SemOpenAlex for { <https://semopenalex.org/work/W2035490018> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2035490018 endingPage "6582" @default.
- W2035490018 startingPage "6573" @default.
- W2035490018 abstract "We have developed an analytical method, consisting of ion-pair liquid chromatography coupled to electrospray ionization mass spectrometry (IP-LC-ESI-MS), for the simultaneous quantitative analysis of several key classes of polar metabolites, like nucleotides, coenzyme A esters, sugar nucleotides, and sugar bisphosphates. The use of the ion-pair agent hexylamine and optimization of the pH of the mobile phases were critical parameters in obtaining good retention and peak shapes of many of the above-mentioned polar and acidic metabolites that are impossible to analyze using standard reversed-phase LC/MS. Optimum conditions were found when using a gradient from 5 mM hexylamine in water (pH 6.3) to 90% methanol/10% 10 mM ammonium acetate (pH 8.5). The IP-LC-ESI-MS method was extensively validated by determining the linearity (R2 > 0.995), sensitivity (limit of detection 0.1−1 ng), repeatability, and reproducibility (relative standard deviation <10%). The IP-LC-ESI-MS method was shown to be a useful tool for microbial metabolomics, i.e., the comprehensive quantitative analysis of metabolites in extracts of microorganisms, and for the determination of the energy charge, i.e., the cellular energy status, as an overall quality measure for the sample workup and analytical protocols." @default.
- W2035490018 created "2016-06-24" @default.
- W2035490018 creator A5044095101 @default.
- W2035490018 creator A5075749579 @default.
- W2035490018 creator A5076255429 @default.
- W2035490018 creator A5076591393 @default.
- W2035490018 creator A5084519039 @default.
- W2035490018 creator A5090294435 @default.
- W2035490018 date "2006-07-22" @default.
- W2035490018 modified "2023-10-14" @default.
- W2035490018 title "Simultaneous Quantitative Analysis of Metabolites Using Ion-Pair Liquid Chromatography−Electrospray Ionization Mass Spectrometry" @default.
- W2035490018 cites W1838886870 @default.
- W2035490018 cites W1970131336 @default.
- W2035490018 cites W1972198125 @default.
- W2035490018 cites W1980317106 @default.
- W2035490018 cites W2016968987 @default.
- W2035490018 cites W2027677701 @default.
- W2035490018 cites W2033450743 @default.
- W2035490018 cites W2039410725 @default.
- W2035490018 cites W2043008845 @default.
- W2035490018 cites W2062954396 @default.
- W2035490018 cites W2069426382 @default.
- W2035490018 cites W2069796584 @default.
- W2035490018 cites W2070148925 @default.
- W2035490018 cites W2106444668 @default.
- W2035490018 cites W2110413022 @default.
- W2035490018 cites W2112258048 @default.
- W2035490018 doi "https://doi.org/10.1021/ac0607616" @default.
- W2035490018 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16970336" @default.
- W2035490018 hasPublicationYear "2006" @default.
- W2035490018 type Work @default.
- W2035490018 sameAs 2035490018 @default.
- W2035490018 citedByCount "208" @default.
- W2035490018 countsByYear W20354900182012 @default.
- W2035490018 countsByYear W20354900182013 @default.
- W2035490018 countsByYear W20354900182014 @default.
- W2035490018 countsByYear W20354900182015 @default.
- W2035490018 countsByYear W20354900182016 @default.
- W2035490018 countsByYear W20354900182017 @default.
- W2035490018 countsByYear W20354900182018 @default.
- W2035490018 countsByYear W20354900182019 @default.
- W2035490018 countsByYear W20354900182020 @default.
- W2035490018 countsByYear W20354900182021 @default.
- W2035490018 countsByYear W20354900182022 @default.
- W2035490018 countsByYear W20354900182023 @default.
- W2035490018 crossrefType "journal-article" @default.
- W2035490018 hasAuthorship W2035490018A5044095101 @default.
- W2035490018 hasAuthorship W2035490018A5075749579 @default.
- W2035490018 hasAuthorship W2035490018A5076255429 @default.
- W2035490018 hasAuthorship W2035490018A5076591393 @default.
- W2035490018 hasAuthorship W2035490018A5084519039 @default.
- W2035490018 hasAuthorship W2035490018A5090294435 @default.
- W2035490018 hasConcept C113196181 @default.
- W2035490018 hasConcept C119128265 @default.
- W2035490018 hasConcept C154020017 @default.
- W2035490018 hasConcept C162356407 @default.
- W2035490018 hasConcept C175467004 @default.
- W2035490018 hasConcept C179998833 @default.
- W2035490018 hasConcept C185592680 @default.
- W2035490018 hasConcept C194451485 @default.
- W2035490018 hasConcept C2780782300 @default.
- W2035490018 hasConcept C40684141 @default.
- W2035490018 hasConcept C43617362 @default.
- W2035490018 hasConcept C64489805 @default.
- W2035490018 hasConcept C95986675 @default.
- W2035490018 hasConceptScore W2035490018C113196181 @default.
- W2035490018 hasConceptScore W2035490018C119128265 @default.
- W2035490018 hasConceptScore W2035490018C154020017 @default.
- W2035490018 hasConceptScore W2035490018C162356407 @default.
- W2035490018 hasConceptScore W2035490018C175467004 @default.
- W2035490018 hasConceptScore W2035490018C179998833 @default.
- W2035490018 hasConceptScore W2035490018C185592680 @default.
- W2035490018 hasConceptScore W2035490018C194451485 @default.
- W2035490018 hasConceptScore W2035490018C2780782300 @default.
- W2035490018 hasConceptScore W2035490018C40684141 @default.
- W2035490018 hasConceptScore W2035490018C43617362 @default.
- W2035490018 hasConceptScore W2035490018C64489805 @default.
- W2035490018 hasConceptScore W2035490018C95986675 @default.
- W2035490018 hasIssue "18" @default.
- W2035490018 hasLocation W20354900181 @default.
- W2035490018 hasLocation W20354900182 @default.
- W2035490018 hasOpenAccess W2035490018 @default.
- W2035490018 hasPrimaryLocation W20354900181 @default.
- W2035490018 hasRelatedWork W1889713604 @default.
- W2035490018 hasRelatedWork W2005493133 @default.
- W2035490018 hasRelatedWork W2029217370 @default.
- W2035490018 hasRelatedWork W2078470673 @default.
- W2035490018 hasRelatedWork W2096722395 @default.
- W2035490018 hasRelatedWork W2545638916 @default.
- W2035490018 hasRelatedWork W2604342365 @default.
- W2035490018 hasRelatedWork W2606359044 @default.
- W2035490018 hasRelatedWork W2739418042 @default.
- W2035490018 hasRelatedWork W2950115409 @default.
- W2035490018 hasVolume "78" @default.
- W2035490018 isParatext "false" @default.
- W2035490018 isRetracted "false" @default.
- W2035490018 magId "2035490018" @default.
- W2035490018 workType "article" @default.